FTC-238

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 94060902, human thyroid (lung metastasis), Polymorphic

• Code UNSPSC : 41106514

Propriétés

• Source biologique: human thyroid (lung metastasis)
• Conditionnement: tube of 5 μg 94060902-DNA-5UG; pkg of vial of cells 94060902-1VL
• Mode de croissance: Adherent
• Caryotype: Not specified
• Morphologie: Polymorphic
• Produits: Expression of 5′ - Deiodinase Type I
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): metastasis
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human follicular thyroid carcinoma, lung metastasis

Description de la lignée cellulaire

FTC-238 was established from a lung metastasis of a follicular thyroid carcinoma from a 42-year-old male. The cells are polymorphic showing flat polygonal to spindle-like morphologies. Complex chromosomal changes were detected as well as a p53 mutation. FTC-133 (Sigma Catalogue number 94060901), FTC-236 (Sigma Catalogue number 06030202) and FTC-238 (Sigma Catalogue number 94060902) were all derived from the same individual, a 42 year old male with follicular thyroid cancer. This has been confirmed by short tandem repeat STR-PCR analysis at ECACC The Y chromosome could not be detected in these cell line by (STR)-PCR analysis. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell lines are identical to the source provided by the depositor based on the STR-PCR analysis.

Application

FTC-238 has been used to study the function of human thyroid and development of thyroid cancer.[1][2]

Profil d'ADN

STR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 11
D16S539: 11,12
D5S818: 11,12
D7S820: 9,10
THO1: 9.3
TPOX: 9
vWA: 15,18

Milieu de culture

DMEM:Ham′s F12 (1:1) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:10 to 1:20 i.e. seeding at 1-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Autres remarques

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