EJG

93051803, bovine adrenal gland, Endothelial

• Code UNSPSC : 41106514

Propriétés

• product name: EJG, 93051803
• Source biologique: bovine adrenal gland
• Mode de croissance: Adherent
• Caryotype: Not specified
• Morphologie: Endothelial
• Produits: Factor VIII
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Bovine adrenal medulla endothelial

Description de la lignée cellulaire

The bovine cell line was established from collagenase-dissociated, adrenal medulla tissue. The cells display a capillary endothelium morphology with relatively large cell bodies and produce factor VIII:C. Tests for BVDV have shown that the cell line is producing both detectable BVDV antigens and infectious BVDV.

Application

Blood clotting studies

Milieu de culture

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. Passage cells every 5 to 7 days.

Autres remarques

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