OX-82

13082213, from Mouse x Mouse Hybridoma

• Code UNSPSC : 41106514

Propriétés

• Source biologique: Mouse x Mouse Hybridoma
• ImmunoDon: BALB/c mouse spleen
• Myélome: NS1
• Réactivité: Rat neutrophils, erythrocyte stem cells, 50% bone marrow
• Technique(s): cell culture | mammalian: suitable
• Isotype: IgG1
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Description de la lignée cellulaire

The hybridoma cell line OX-82 secretes monoclonal IgG1 antibodies reactive with an uncharacterised neutrophil marker.

Immunogène

Rat bone marrow cells

Application

Detection of neutrophils.

Milieu de culture

RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS)

Procédure de repiquage

Seed at 5 x 10⁴ cells/ml (5 x 10⁵ cells/ml at resuscitation). Subculture when cells reach 5 x 10⁵ - 1 x 10⁶ cells/ml (usually after 3-4 days), 5% CO₂, 37°C. 20% FBS can be used at resuscitation.

Autres remarques

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Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.