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C-44010

Sigma-Aldrich

Lymphocyte Separation Medium 1077

500 ml

Synonyme(s) :

Lymphocyte Medium, Separation Medium for Lymphocytes

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500 ML
116.00 CHF

116.00 CHF

Date d'expédition estimée le15 mai 2025

Devis pour commande en gros
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500 ML
116.00 CHF

About This Item

Code UNSPSC :
12352207
Nomenclature NACRES :
NA.71

116.00 CHF

Date d'expédition estimée le15 mai 2025

Devis pour commande en gros

Forme

aqueous solution

Conditionnement

pkg of 500 ml

Technique(s)

cell culture | mammalian: suitable

Conditions d'expédition

ambient

Température de stockage

room temp

Description générale

Lymphocyte Separation Medium 1077

Application

Lymphocyte Separation Medium 1077 is intended for the separation of the portion of vital mononuclear cells from whole blood, buffy coats, bone marrow and several other starting materials, e.g. crude cell preparations, by means of low density gradient centrifugation. Lymphocyte separation Medium has a density of 1.077 g/ml at 20°C. It is sterile, ready-to use and based on Ficoll 400 and sodium diatrizoate providing optimal physiological parameters and low cytotoxicity.

Qualité

All lots of PromoCell Lymphocyte Separation Medium 1077 are subjected to comprehensive quality control tests. Each lot is routinely tested for function and physical parameters such as osmolality and pH level. Approved in-house lots are used as a reference. In addition, all lots have been tested for the absence of microbial contaminants (fungi, bacteria).

Stockage et stabilité

Lymphocyte Separation Medium 1077 is light-sensitive! Store the medium at room temperature in the dark immediately after arrival. Do not freeze. If stored properly, the product is stable until the expiry date stated on the label.

Procédure de repiquage

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Pictogrammes

Corrosion
GHS05

Mention d'avertissement

Warning

Mentions de danger

H290,H315,H319

Classification des risques

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

Code de la classe de stockage

8B - Non-combustible corrosive hazardous materials

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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Certificats d'analyse (COA)

Lot/Batch Number

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Consulter la Bibliothèque de documents

Fengyu Ming et al.
BioMed research international, 2020, 4658109-4658109 (2020-10-09)
Parkinson's disease (PD) is the second most common neurodegenerative disease in middle-aged and elderly people. However, the etiology and pathogenesis of PD are still unclear and there is a lack of reliable biomarkers for early molecular diagnosis. Parkin (encoded by

Articles

Primary Human Mononuclear Cells Culture

Umbilical cord blood and adult peripheral blood mononuclear cells with optimized maintenance medium for short-term cultivation.

Questions

1–2 of 2 Questions  

  1. What's the procedure of lymphocytes separation with this media and the quantity used .

    1 answer

    1. This is a product of PromoCell. Please see the instructions for use provided by the manufacturer:
      1. Fill Lymphocyte Separation Medium 1077 in a tube. Use 3 ml for a 15 ml tube and 20 ml for a 50 ml tube.
      2. Hold the tube at a 45° angle and carefully load the cell suspension to be separated on top of the separation medium.
      Note: Perform this step slowly but constantly. Take care that the two phases do not mix and a sharp border is present.
      For best results, use an electrical pipet aid with EX-function allowing the slow and constant efflux of the cell suspension from the pipet.
      3. Centrifuge the tube at 440 x g for 40 minutes at room temperature without brake.
      4. Carefully aspirate most of the supernatant without disturbing the layer of mononuclear cells in the interphase.
      5. Aspirate the ring of mononuclear cells from the interphase using a pipet. Keep the volume as small as possible.
      6. Wash the mononuclear cells with PBS/0.1% HSA or BSA and centrifuge at 360 x g for 10 minutes.
      7. Wash the mononuclear cells with PBS/0.1% HSA or BSA and centrifuge at 200 x g for 10 minutes X 2
      8. The mononuclear cell preparation is ready.

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  2. What percentage of lymphocytes/monocytes, etc. would be expected if they use it on fresh human blood?

    1 answer

    1. When utilizing the Lymphocyte Separation Medium 1077 (cat. #C-44010) on fresh human whole blood, the expected percentage of lymphocytes/monocytes typical for this sample is usually in the range of 70-90% of the enriched PBMCs.

      Helpful?

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