C-39220
Endothelial Cell Growth Medium MV
SupplementPack containing all media supplements as individual vials, 1 Pack for 500 ml
Synonyme(s) :
Recommended with C-22220
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
About This Item
Code UNSPSC :
12352207
Nomenclature NACRES :
NA.81
Produits recommandés
Forme
liquid
Conditionnement
pkg of 1 kit
Technique(s)
cell culture | mammalian: suitable
Conditions d'expédition
dry ice
Température de stockage
−20°C
Description générale
Endothelial Cell Growth Medium MV SupplementPack (basal medium sold separately)
To form a complete medium, it should be combined with the basal medium: C-22220
To form a complete medium, it should be combined with the basal medium: C-22220
Application
Endothelial Cell Growth Medium MV was developed for the in vitro cultivation of endothelial cells from microvascular vessels, the coronary artery, and the aorta. The media are optimized for primary human cells but can also be used for bovine, porcine, murine, rat and dog endothelial cells from microvascular vessels as well as for various endothelial cell lines.Recommended for:Human Dermal Microvascular Endothelial Cells (HDMEC)Human Coronary Artery Endothelial Cells (HCAEC)Human Aortic Endothelial Cells (HAoEC)Human Pulmonary Microvascular Endothelial Cells (HPMEC)Human Dermal Lymphatic Endothelial Cells (HDLEC)Human Dermal Blood Endothelial Cells (HDBEC)Human Cardiac Microvascular Endothelial Cells (HCMEC)Human Uterine Microvascular Endothelial Cells (HUtMEC)Note: For best transfection results we recommend using PromoCell heparin-free Endothelial Cell Growth Medium MV2. Heparin, included all our other our Endothelial Cell Growth Media formulations, may reduce transfection efficiency.
Composants
Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials
Qualité
All lots of PromoCell Endothelial Cell Media MV/MV2 are subjected to comprehensive quality control tests using primary human endothelial cells. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested endothelial cells. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).
Notes préparatoires
Final supplement concentrations after addition to the basal medium:Fetal Calf Serum: 0.05 ml / mlEndothelial Cell Growth Supplement: 0.004 ml / mlEpidermal Growth Factor (recombinant human): 10 ng / mlHeparin: 90 µg / mlHydrocortisone: 1 µg / ml
Stockage et stabilité
Store the SupplementPack at -20°C in the dark immediately after arrival. If stored properly, it is stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.Note: The SupplementPack is delivered thawed and can be frozen after arrival without losing any activity.
Procédure de repiquage
Click here for more information.
En option
Réf. du produit
Description
Tarif
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Eye Irrit. 2 - Flam. Liq. 2
Code de la classe de stockage
3 - Flammable liquids
Point d'éclair (°F)
55.4 °F
Point d'éclair (°C)
13 °C
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Cuicui Guo et al.
Journal of separation science, 44(16), 3052-3060 (2021-06-09)
Human exposure to bisphenols has rarely been reported. The most important challenges in this regard are the sensitivity and accuracy of the analytical methods employed. Dansyl chloride derivatization prior to high-performance liquid chromatography-tandem mass spectrometry has been prevalently employed to
M Reza Anari et al.
Analytical chemistry, 74(16), 4136-4144 (2002-08-30)
The extensive metabolism and administration of low doses of ethinylestradiol (EE) in preclinical animal species necessitates a sensitive analytical method to quantify the drug at low picogram-per-milliliter concentrations in biological matrixes. A highly sensitive and accurate method based on the
Bianca Patrascu et al.
International journal of molecular sciences, 21(10) (2020-05-24)
Starting from dansyl-chloride, in reaction with 1,1-diphenylhydrazine and methoxyamine, two new fluorescent derivatives 1 and 2 were obtained and characterized by NMR, IR, UV-Vis, HR-MS, and fluorescence spectroscopy. The single-crystal X-ray structure was obtained for compound 2. Both compounds generate
Protocoles
Cell culture protocol: the endothelial cell transwell migration and invasion assay used to study angiogenesis and cancer cell metastasis. Explore over 350 PromoCell products.
Contenu apparenté
Cell culture protocol: the endothelial tube formation assay to study angiogenesis using HUVECs and other endothelial cell types. Explore over 350 PromoCell products.
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique