This medium is a complete, ready-to-use product; no supplementation is required. This formulation contains hydrocortisone, heparin and other growth factors needed to support Endothelial Cell growth. Unless a suspension culture is required, the culture vessel should be tissue culture treated. The additional use of attachment matrices such as gelatin, would be dependent on the requirements of the specific cell line or type.
211-500
Endothelial Cell Growth Medium (500 ml)
Synonyme(s) :
Primary Cell Culture Media
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About This Item
Code UNSPSC :
12352207
Nomenclature NACRES :
NA.71
Le tarif et la disponibilité ne sont pas disponibles actuellement.
Produits recommandés
Forme
liquid
Niveau de qualité
Durée de conservation
6 mo.
Fabricant/nom de marque
Cell Applications, Inc
Conditions d'expédition
wet ice
Température de stockage
2-8°C
Description générale
All-in-one ready-to-use media
Endothelial Cell Growth Medium (500 ml) is an all-in-one ready-to-use. Optimized and best-suited for angiogenesis and other physiological studies.
Application
Qualité
Each lot was tested for the ability to support plating, spreading and proliferation
Notes préparatoires
contained in protocol
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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Articles
Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.
Protocoles
Technical information for working with human pulmonary artery endothelial cells including thawing, subculturing and cryopreservation
Technical information for working with human aortic endothelial cells including thawing, subculturing and cryopreservation.
Human Umbilical Vein Endothelial Cells (HUVEC) Culture Protocol. Storage. Preparation for Culturing. Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.
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If I use this medium, do I need to use tissue coated treated flasks with gelatin? Does this medium need to be supplemented with heparin or is it already included?
1 answer-
Helpful?
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Can you please provide the full composition of the medium with the concentrations?
1 answer-
This item is a product of Cell Applications. The formulation is considered proprietary. However, this medium does include glucose at 1g/L, 2% Fetal Bovine Serum, as well as Penicillin, streptomycin, and Amphotericin B. Some salt components have also been published in the Safety Data Sheet.
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Do you sell glucose-free ECGM? I needed it for HUVEC cells. 0mM glucose.
1 answer-
This product is not a glucose-free medium. The formulation contains 5.78 mM glucose which is 5.55 mM after all the supplements are added. Unfortunately, a glucose-free ECGM medium is not currently offered.
Helpful?
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