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C2010

Sigma-Aldrich

Concanavalin A from Canavalia ensiformis (Jack bean)

Type IV, lyophilized powder

Synonyme(s) :

Con A

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About This Item

Numéro CAS:
11028-71-0
Numéro CE :
234-258-2
Numéro MDL:
MFCD00071069
Code UNSPSC :
23151817
Nomenclature NACRES :
NA.32

Type

Type IV

Forme

lyophilized powder

Puissance

<64 μg per mL agglutination activity

Impuretés

Carbohydrate, essentially free
 salt, essentially free

Température de stockage

−20°C

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Catégories apparentées

Description générale

Concanavalin A (con A) is a plant lectin refined from jack beans. It is a tetrameric protein, which has four identical 28 kDa subunits. Con A has the ability to connect with the mannose residues of several glycoproteins and can stimulate lymphocytes.

Application

Concanavalin A from Canavalia ensiformis (Jack bean) has been used:
  • as T-lymphocyte mitogen in lymphocyte proliferation assay for correlations with organochlorine contaminants (OCs)
  • for in vitro stimulation of T cell hybridomas, BW5147 cells and primary CD4+ T cells for T cell vaccination and for induction of hepatitis
  • for the stimulation of mice splenocytes in the suppression of T-lymphocytes proliferation assay
  • for the stimulation of spleen in mitogen assays

Actions biochimiques/physiologiques

Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.

Qualité

Highly purified by specific affinity chromatography

Remarque sur l'analyse

Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca2+ and Mn2+ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Danger

Mentions de danger

H317,H334,H361

Classification des risques

Repr. 2 - Resp. Sens. 1 - Skin Sens. 1

Code de la classe de stockage

13 - Non Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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J L Barnett et al.
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Prud'Homme G J, et al.
The Journal of Experimental Medicine, 159(2), 463-478 (1984)
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Han Y
International Immunopharmacology, 9(2), 207-211 (2009)
Elucidating kinetic and thermodynamic constants for interaction of G protein subunits and receptors by surface plasmon resonance spectroscopy.
Rebois R V, et al.
Test, 344, 15-15 (2002)
Aditi Varthaman et al.
Journal of immunology (Baltimore, Md. : 1950), 184(12), 6585-6591 (2010-05-22)
Administration of attenuated pathogenic T cell clones, a procedure known as T cell vaccination, induces CD8+ T cells specific for peptides derived from the Vbeta-chain of the TCR presented by the MHC class Ib molecule, Qa-1 expressed on the vaccine
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