A2980
Anti-AcV5 antibody, Mouse monoclonal
clone ACV5, purified from hybridoma cell culture
Synonyme(s) :
Anti-Baculovirus envelope gp64 protein
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About This Item
Produits recommandés
Source biologique
mouse
Niveau de qualité
Conjugué
unconjugated
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
ACV5, monoclonal
Forme
buffered aqueous solution
Poids mol.
antigen ~58 kDa
Conditionnement
antibody small pack of 25 μL
Concentration
~1 mg/mL
Technique(s)
dot blot: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: 0.5-1 μg/mL using extracts of Sf9 cells infected with baculovirus
Isotype
IgG2b
Conditions d'expédition
dry ice
Température de stockage
−20°C
Modification post-traductionnelle de la cible
unmodified
Description générale
Anti-AcV5 antibody, Mouse monoclonal, (mouse IgG2b isotype) is derived from the hybridoma ACV5 produced by the fusion of mouse myeloma cells and splenocytes from C57Bl/6 X Balb/c mice immunized with AcNPV extracellular nonoccluded virus (NOV). GP64 (AcV5) is an envelope glycoprotein found in the baculovirus Autographa californica multiple nucleopolyhedrovirus. GP64 gene open reading frame encodes a protein of 509 amino acids with a molecular weight of 58 kDa. The protein contains N-linked glycosylation sites and hydrophobic N- and C-termini, characteristic of signal and membrane anchor motifs found in envelope glycoproteins. The GP64 protein is present early (6 hr) post infection and accumulates in the infected cell moving to the periphery later.
gp64 (AcV5) is an envelope glycoprotein found in the baculovirus Autographa californica multiple nucleopolyhedrovirus. It is involved in involved in viral entry into cells by receptor-mediated endocytosis. Elevated expression of gp64 has been shown to enhance gene transfer in a variety of cell types.
Immunogène
AcNPV extracellular nonocculeded virus (NOV).
Application
Anti-AcV5 antibody, Mouse monoclonal has been used in:
- immunoblotting
- immunoprecipitation
- dot blot
- immunocytochemistry
Proteins tagged with this AcV5 peptide are functional in both dicot and monocot plants and show no cross reactivity across many plant species. Epitope tagged fusion proteins are widely used in protein analysis of biological samples (e.g. plant cell extracts). Antibodies specific for the tag are a valuable means for the detection of the protein. Anti-AcV5 antibody, Mouse monoclonal, clone ACV5 may be used in immunoblotting, immunoprecipitation, dot blot, and immunocytochemistry.
Actions biochimiques/physiologiques
GP64 (AcV5) is involved in viral entry into cells by receptor-mediated endocytosis. Elevated expression of gp64 has been shown to enhance gene transfer in a variety of cell types. AcV5 is a monoclonal antibody that recognizes a nine amino acid residue tag (SWKDASGWS) of baculovirus Autographa californica GP64 envelope fusion protein (efp).
Forme physique
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Shudi Zhao et al.
Frontiers in immunology, 13, 845268-845268 (2022-03-08)
Antiviral immunity involves various mechanisms and responses, including the RNA interference (RNAi) pathway. During long-term coevolution, viruses have gained the ability to evade this defense by encoding viral suppressors of RNAi (VSRs). It was reported that p35 of baculovirus can
Location, sequence, transcriptional mapping, and temporal expression of the gp64 envelope glycoprotein gene of the Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus
Blissard GW and Rohrmann GF
Virology, 170(2), 537-555 (1989)
Boris F Mayer et al.
Plant physiology, 182(2), 1022-1038 (2019-12-18)
Anthropogenic climate change precipitates the need to understand plant adaptation. Crucial in temperate climates, adaptation to winter is characterized by cold acclimation and vernalization, which respectively lead to freezing tolerance and flowering competence. However, the progression of these responses during
Autographa californica multiple nucleopolyhedrovirus GP64 protein: roles of histidine residues in triggering membrane fusion and fusion pore expansion
Li Z and Blissard GW
Journal of virology, 85(23), 12492-12504 (2011)
Baculovirus GP64-mediated entry into mammalian cells
Kataoka C, et al.
Journal of virology, 86(5), 2610-2620 (2012)
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