This kit is a complete, ready-to-use microtiter plate assay kit for the colorimetric detection of proteins. It is based on the reducing reaction of a tetrazolium salt under basic conditions. Tetrazolium salt is easily reduced with sugar residues of proteins and generates formazan dye. The maximum wavelength of the WST-8 formazan dye is 650 nm at pH 12.5. The detection range of this protein quantification kit is from 50 μg/ml to 5000 μg/ml (incubation time 30 min).
Composants
Contents: WST-8 Solution 10 ml, Buffer Solution 100 ml, BSA Standard Solution (10 mg/ml) 1.5 mL (500 tests)
Composants de kit seuls
Réf. du produit
Description
WST-8 Solution 10 mL
Buffer Solution 100 mL
BSA Standard Solution (10 mg/ml) 1.5 mL
Code de la classe de stockage
10 - Combustible liquids
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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Analytical and bioanalytical chemistry, 399(1), 347-352 (2010-11-05)
In this study, we give the proof of concept for a method to determine binding constants of compounds in solution. By implementing a technique based on magnetic beads with a microfluidic device for segmented flow generation, we demonstrate, for individual
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