52403
Anti-Mouse IgG−Abberior® STAR 580 antibody produced in goat
for STED application
Synonyme(s) :
Abberior® STAR 580-Anti-Mouse-IgG antibody produced in goat
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About This Item
Code UNSPSC :
12352200
Nomenclature NACRES :
NA.32
Produits recommandés
Source biologique
goat
Forme d'anticorps
affinity isolated antibody
Type de produit anticorps
secondary antibodies
Clone
polyclonal
Forme
buffered aqueous solution
Espèces réactives
mouse
Disponibilité
available only in USA and Canada
Concentration
~1 mg/mL
Fluorescence
λex 587 nm; λem 607 nm in PBS, pH 7.4
Température de stockage
−20°C
Description générale
Abberior STAR 580 was developed for STED and confocal microscopy in the orange spectral region. Abberior STAR 580 is highly photostable and bright orange fluorescent dye. The dye works extremely well with Abberior Instruments STEDYCON and Expert Line microscopes and Leica STED microscopes. It can be very effectively excited with excitation wavelength between 550 - 600 nm. Abberior STAR 580 can substitute dyes like Cy3™, Atto™ 590 or Alexa Fluor™ 568. For STED microscopy, Abberior STAR 580 can be used most efficiently with a STED Laser wavelength between 750 - 800 nm. Abberior STAR 580 is the ideal partner for Abberior STAR RED or Abberior STAR 635P to obtain optimal 2 Color STED results. Best results are obtained with freshly prepared samples.
Photophysical properties (carboxylic acid):
Absorption Maximum, λex [nm]: 587 (PBS pH 7.4), 584 (H2O), 584 (MeOH)
Extinction Coefficient, εmax [M-1cm-1]: 85 000 (PBS pH 7.4), 85 000 (H2O), 95 000 (MeOH)
Correction Factor, CF260 = ε260/εmax: 0,17 (PBS pH 7.4)
Correction Factor, CF280 = ε280/εmax: 0,17 (PBS pH 7.4)
Fluorescence Maximum, λem [nm]: 607 (PBS pH 7.4), 607 (H2O), 604 (MeOH)
Recommended STED Wavelength, λ [nm]: 750-800
Fluorescence Quantum Yield, λ: 0,90 (PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 3,5 (PBS pH 7.4)
Photophysical properties (carboxylic acid):
Absorption Maximum, λex [nm]: 587 (PBS pH 7.4), 584 (H2O), 584 (MeOH)
Extinction Coefficient, εmax [M-1cm-1]: 85 000 (PBS pH 7.4), 85 000 (H2O), 95 000 (MeOH)
Correction Factor, CF260 = ε260/εmax: 0,17 (PBS pH 7.4)
Correction Factor, CF280 = ε280/εmax: 0,17 (PBS pH 7.4)
Fluorescence Maximum, λem [nm]: 607 (PBS pH 7.4), 607 (H2O), 604 (MeOH)
Recommended STED Wavelength, λ [nm]: 750-800
Fluorescence Quantum Yield, λ: 0,90 (PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 3,5 (PBS pH 7.4)
Application
Anti-Mouse IgG-Abberior® STAR 580 antibody produced in goat has been used for STED (stimulated emission depletion) microscopy in CA3 (Cornus Ammonis) sections.
Adéquation
Designed and tested for fluorescent super-resolution microscopy
Remarque sur l'analyse
May contain significant amounts of BSA as stabilizer
unconjugated dye ≤5% of total fluorescence
unconjugated dye ≤5% of total fluorescence
Autres remarques
Informations légales
ALEXA FLUOR is a trademark of Life Technologies
Atto is a trademark of Atto-Tec GmbH
Cy3 is a trademark of Cytiva
abberior is a registered trademark of Abberior GmbH
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Produit(s) apparenté(s)
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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