This product is lyophilized from a solution containing phosphate buffer and sucrose and may be reconstituted in water. For testing purposes, this product is dissolved at 5 mg/mL in a cold assay buffer. See below to review the composition of the 50 mM Tris assay buffer:
1.0 ml 1 M Tris stock solution
0.4 ml of 0.5 M magnesium chloride stock solution
0.01 ml Mercapto-ethanol
Add to 15 ml H2O
adjust pH 7.3 with HCl 1 M
QS to 20 ml
48275
β-Galactosidase from Escherichia coli
suitable for enzyme immunoassay, lyophilized, powder, ~140 U/mg
Synonyme(s) :
β-D-Galactoside galactohydrolase, Lactase
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Sélectionner une taille de conditionnement
1 MG
46.10 CHF
5 MG
159.00 CHF
25 MG
403.00 CHF
46.10 CHF
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Sélectionner une taille de conditionnement
Changer de vue
1 MG
46.10 CHF
5 MG
159.00 CHF
25 MG
403.00 CHF
About This Item
46.10 CHF
Check Cart for Availability
Produits recommandés
Forme
powder
Qualité
lyophilized
Activité spécifique
~140 U/mg
Poids mol.
Mr ~135000
Contient
phosphate buffer as stabilizer
sucrose as stabilizer
Technique(s)
enzyme immunoassay: suitable
Conditions d'expédition
wet ice
Température de stockage
−20°C
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Catégories apparentées
Application
Aqueous solution may be used directly for derivatization without previous dialysis or gel permeation chromatography.
Actions biochimiques/physiologiques
βLa β-galactosidase clive le lactose en ses constituants monosaccharides, le glucose et le galactose. Elle catalyse également la transglycosylation du glucose en allolactose, inducteur de la β-galactosidase, dans une boucle de rétroaction.
Conditionnement
Bottomless glass bottle. Contents are inside inserted fused cone.
Propriétés physiques
Poids moléculaire du tétramère : 465 kDa (chaque sous-unité ayant 116,3 kDa)
Définition de l'unité
1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol 2-nitrophenyl-β-D-galactopyranoside (Cat. No. 73660) per minute at pH 7.8 and 37 °C
Autres remarques
Used in a highly sensitive enzyme-linked immunoassay of human IgG with 4-methylumbelliferyl-β-D-galactoside (Catalog No. 69590)citation; Evaluation of β-D-galactosidase (E. coli) and horse radish peroxidase as labels by sandwich enzyme immunoassay techniquecitation; Enzyme immunoassay of thyrptropin releasing hormone (TRH)citation; Preparation of enzyme-antibody conjugatescitation
Used in a highly sensitive enzyme-linked immunoassay of human IgG with 4-methylumbelliferyl-β-D-galactoside (Catalog No. 69590)[1]; Evaluation of β-D-galactosidase (E. coli) and horse radish peroxidase as labels by sandwich enzyme immunoassay technique[2]; Enzyme immunoassay of thyrptropin releasing hormone (TRH)[3]; Preparation of enzyme-antibody conjugates[4]
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
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Les clients ont également consulté
M Imagawa et al.
Annals of clinical biochemistry, 21 ( Pt 4), 310-317 (1984-07-01)
beta-D-galactosidase from Escherichia coli and horseradish peroxidase were evaluated as labels of Fab' in dose-response curves for human alpha-fetoprotein and human chorionic gonadotropin by sandwich enzyme immunoassay technique using fluorogenic substrates for enzyme assay. The non-specific binding of Fab'-peroxidase conjugates
Comparison of two methods of preparing enzyme-antibody conjugates: application of these conjugates for enzyme immunoassay.
M J O'Sullivan et al.
Analytical biochemistry, 100(1), 100-108 (1979-11-15)
K Kato et al.
European journal of biochemistry, 62(2), 285-292 (1976-02-16)
Rabbit immunoglobulin G (IgG) was reduced by incubating with 10 mM 2-mercaptoethylamine and then treated with excess amounts of N,N'-o-phenylenedimaleimide. As a result, maleimide residues were introduced into rabbit IgG molecules. Rabbit IgG containing maleimide residues could be coupled to
M Takeyama et al.
Chemical & pharmaceutical bulletin, 40(8), 2199-2201 (1992-08-01)
A sensitive and specific double-antibody enzyme immunoassay (EIA) for a thyrotropin releasing hormone (TRH)-like immunoreactive substance has been developed. In order to synthesize TRH-labeled beta-D-galactosidase (beta-gal), a newly devised TRH derivative, pGlu-His-Pro-NH-(CH2)6-NH2 (TRH-Hex), was employed. TRH-Hex was linked to beta-gal
A Hinek et al.
The Journal of clinical investigation, 91(3), 1198-1205 (1993-03-01)
We and others have previously shown that a 67-kD cell surface elastin/laminin-binding protein (EBP) is responsible for cell adhesion to elastin and laminin and for mediating the process of elastin fiber assembly, but the nature of this protein was unknown.
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I have already bought this one. My question is how will I reconstitute it? What diluent or buffer will I use?
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