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45245

Sigma-Aldrich

Eosin 5-isothiocyanate

≥95.0% (UV)

Synonyme(s) :

EITC

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About This Item

Formule empirique (notation de Hill):
C21H7Br4NO5S
Numéro CAS:
60520-47-0
Poids moléculaire :
704.96
Numéro MDL:
MFCD00082502
Code UNSPSC :
12352200
ID de substance PubChem :
57650268

Pureté

≥95.0% (UV)

Fluorescence

λex 521; λem 544 (pH 9.0)

Température de stockage

2-8°C

Chaîne SMILES 

OC(=O)c1cc(ccc1C2=C3C=C(Br)C(=O)C(Br)=C3Oc4c(Br)c(O)c(Br)cc24)N=C=S

InChI

1S/C21H7Br4NO5S/c22-12-4-10-14(8-2-1-7(26-6-32)3-9(8)21(29)30)11-5-13(23)18(28)16(25)20(11)31-19(10)15(24)17(12)27/h1-5,27H,(H,29,30)

Clé InChI

MCXYFYWNOWPDMA-UHFFFAOYSA-N

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Application

Eosin 5-isothiocyanate (EITC) is a fluorescence probe. EITC tagged molecules may be used together with fluorescein-5′-isothiocyanate (FITC) tagged molecules in fluoresce resonance energy transfer (FRET) based assays.

Autres remarques

Acceptor fluorophore. Measurement of rotational diffusion of proteins; In fluorescence energy transfer studies

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Danger

Mentions de danger

H317 - H334

Conseils de prudence

P261 - P280 - P342 + P311

Classification des risques

Resp. Sens. 1 - Skin Sens. 1

Code de la classe de stockage

13 - Non Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Valeria Levi et al.
Biochimica et biophysica acta, 1599(1-2), 141-148 (2002-12-14)
Self-association of bovine serum albumin (BSA) was explored using fluorescence resonance energy transfer (FRET) between two populations of the protein labeled separately with either fluorescein-5'-isothiocyanate (FITC) or eosin-5'-isothiocyanate (EITC). The energy transfer reached the steady state after 5 s at
T Chiba et al.
Biochimica et biophysica acta, 897(1), 14-24 (1987-02-12)
The characteristics of the anion transport system in human erythrocyte, which can be modified by eosin 5-isothiocyanate (EITC), were studied using the pH titration method and by measuring the sulfate efflux. Based on the pH dependence of EITC binding to
S Q Liu et al.
The American journal of physiology, 264(5 Pt 1), C1155-C1164 (1993-05-01)
Although eosin-5-maleimide (EM) covalently labels band 3 and has been thought to react at the external-facing anion transport site, EM reversibly inhibits Cl- exchange at 0 degrees C in a noncompetitive fashion, indicating that under these conditions it does not
Ryan R Hansen et al.
Analytical and bioanalytical chemistry, 392(1-2), 167-175 (2008-07-29)
Quantitative evaluation of minimal polynucleotide concentrations has become a critical analysis among a myriad of applications found in molecular diagnostic technology. Development of high-throughput, nonenzymatic assays that are sensitive, quantitative and yet feasible for point-of-care testing are thus beneficial for
T Chiba et al.
Biochimica et biophysica acta, 858(1), 107-117 (1986-06-13)
The amino-acid residues of band 3 protein taking part in the ionic interaction with an anion-transport inhibitor, eosin 5-isothiocyanate (EITC), were determined by pH titration. The plots of the absorbance of EITC-ghost system against pH reveal five equilibria, at pH
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