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I8377

Sigma-Aldrich

Iodonitrotetrazolium chloride

Used in colorimetric assays.

Synonyme(s) :

2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride, p-Iodonitrotetrazolium Violet, INT

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About This Item

Formule empirique (notation de Hill):
C19H13ClIN5O2
Numéro CAS:
146-68-9
Poids moléculaire :
505.70
Numéro Beilstein :
4093224
Numéro CE :
205-676-2
Numéro MDL:
MFCD00011961
Code UNSPSC :
12171500
ID de substance PubChem :
24896138
Nomenclature NACRES :
NA.47

Niveau de qualité

200

Pureté

≥98% (TLC)

Forme

powder

Pf

240 °C (dec.) (lit.)

Solubilité

H2O: 4 mg/mL

Application(s)

diagnostic assay manufacturing
hematology
histology

Température de stockage

2-8°C

Chaîne SMILES 

[Cl-].[O-][N+](=O)c1ccc(cc1)-[n+]2nc(nn2-c3ccc(I)cc3)-c4ccccc4

InChI

1S/C19H13IN5O2.ClH/c20-15-6-8-16(9-7-15)23-21-19(14-4-2-1-3-5-14)22-24(23)17-10-12-18(13-11-17)25(26)27;/h1-13H;1H/q+1;/p-1

Clé InChI

JORABGDXCIBAFL-UHFFFAOYSA-M

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Application

Iodonitrotetrazolium chloride has been used for the staining of cells. It has been used as a coupling agent in the enzymatic assay of D-arabinitol.
Electron acceptor for the colorimetric assay of various dehydrogenases

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Pictogrammes

FlameExclamation mark
GHS02,GHS07

Mention d'avertissement

Danger

Mentions de danger

H228,H335

Classification des risques

Flam. Sol. 1 - STOT SE 3

Organes cibles

Respiratory system

Code de la classe de stockage

4.1B - Flammable solid hazardous materials

Classe de danger pour l'eau (WGK)

WGK 3

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Certificats d'analyse (COA)

Lot/Batch Number
0000284108
0000310220
SLCQ5874
SLCL0301
SLBZ3933

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Constitutive activation of pp125fak in newly isolated human breast cancer cell lines.
Ignatoski KMW and Ethier SP
Breast Cancer Research and Treatment, 54, 173-173 (1999)
Yang Hongwei et al.
Ecotoxicology and environmental safety, 59(2), 249-255 (2004-08-26)
Anaerobic biodegradability of organic compounds can be assessed from the changes in organic compounds, the end products, and the activity of microorganisms. In this study, all three of these variables were considered to integrate assessment of the anaerobic biodegradability of
Sergio Ravasio et al.
Biochemistry, 41(25), 8120-8133 (2002-06-19)
The properties of the recombinant ferredoxin-dependent glutamate synthase of Synechocystis PCC6803 were determined by means of kinetic and spectroscopic approaches in comparison to those exhibited by the bacterial NADPH-dependent enzyme form. The ferredoxin-dependent enzyme was found to be similar to
Xiao-mei Xie et al.
Journal of environmental sciences (China), 16(2), 252-255 (2004-05-13)
The impact of triazophos, bensulfuron-methyl, chlobenthiazone on soil biochemical characteristics in a paddy soil under controlled moisture (flooded soil) and temperature (25 degrees C) condition was studied. The electron transport system (ETS)/dehydrogenase activity displayed a negative correlation with triazophos, bensulfuron-methyl
Cheng Luo et al.
Journal of biochemical and biophysical methods, 68(2), 101-111 (2006-06-03)
A sensitive spectrophotometric assay for determining mitochondrial malate dehydrogenase activity is described. The assay measures NADH production by coupling it to the reduction of 2-(p-iodophenyl)-3(p-nitrophenyl)-5-phenyl tetrazolium chloride (INT). Via an intermediate electron carrier, either phenazine methosulfate or lipoamide dehydrogenase, INT
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