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D9564

Sigma-Aldrich

DAPI, dilactate

DAPI, dilactate
1 of 1 reviewers received a sample product or took part in a promotion

≥98% purity (HPLC), powder

Synonyme(s) :

4′,6-Diamidino-2-phenyindole, dilactate

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About This Item

Formule empirique (notation de Hill):
C16H15N5 · 2C3H6O3
Poids moléculaire :
457.48
Numéro MDL:
MFCD00467366
Code UNSPSC :
12171500
ID de substance PubChem :
329798905
Nomenclature NACRES :
NA.47
Le tarif et la disponibilité ne sont pas disponibles actuellement.

Nom du produit

DAPI, dilactate, ≥98% (HPLC)

Niveau de qualité

300

Essai

≥98% (HPLC)

Forme

powder

Technique(s)

titration: suitable

Couleur

yellow

Solubilité

H2O: 20 mg/mL

ε (coefficient d'extinction)

28.00-32.50 at 345-351 nm in methanol

Application(s)

diagnostic assay manufacturing
hematology
histology

Température de stockage

2-8°C

Chaîne SMILES 

CC(O)C(O)=O.CC(O)C(O)=O.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N

InChI

InChI=1S/C16H15N5.C3H6O3/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;1-2(4)3(5)6/h1-8,21H,(H3,17,18)(H3,19,20);2,4H,1H3,(H,5,6)

Clé InChI

LCAUPANIXBMNIA-UHFFFAOYSA-N

Description générale

DAPI is an indolenine dye. It carries two amidine components, one on a pendant phenyl ring and another directly on the indole moiety.

Application

DAPI, dilactate has been used for the nuclear staining in cells.[1][2] DAPI is a common flow cytometry stain that is widely used as a DNA and nuclear stain. Additionally, it is used as a counterstain following in situ hybridization and other immunofluorescence procedures.

Actions biochimiques/physiologiques

A high sensitivity dye used to detect single nucleic acid molecules. DAPI has been shown to detect a single DNA molecule in solution, and to detect femtograms of DNA in single cells and chloroplasts.

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

08168

Timestrip Plus™ 8 °C

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

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Certificats d'analyse (COA)

Lot/Batch Number
0000406681
0000406681
0000384458
0000384458
0000352352

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Shahrzad Bahrampour et al.
PLoS biology, 17(2), e3000163-e3000163 (2019-02-27)
During central nervous system (CNS) development, genetic programs establish neural stem cells and drive both stem and daughter cell proliferation. However, the prominent anterior expansion of the CNS implies anterior-posterior (A-P) modulation of these programs. In Drosophila, a set of
Expression and high glucose-mediated regulation of K+ channel interacting protein 3 (KChIP3) and KV4 channels in retinal Muller glial cells.
Chavira-Suarez E, et al.
Biochemical and Biophysical Research Communications, 404, 678-683 (2011)
Vicky Goler-Baron et al.
PloS one, 6(1), e16007-e16007 (2011-02-02)
Multidrug resistance (MDR) is a major impediment to curative cancer chemotherapy. The ATP-Binding Cassette transporters ABCG2, ABCB1 and ABCC2 form a unique defense network against multiple structurally and functionally distinct chemotherapeutics, thereby resulting in MDR. Thus, deciphering novel mechanisms of
Nicolas Buchon et al.
BMC biology, 8, 152-152 (2010-12-24)
Gut homeostasis is central to whole organism health, and its disruption is associated with a broad range of pathologies. Following damage, complex physiological events are required in the gut to maintain proper homeostasis. Previously, we demonstrated that ingestion of a
Transplantation reveals regional differences in oligodendrocyte differentiation in the adult brain.
Vigano F, et al.
Nature Neuroscience, 16, 1370-1372 (2013)
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Questions

1–4 of 4 Questions  

  1. Which filter sets can be used to visualize the fluorophore produced by Product D9564, DAPI, dilactate (DAPI-DNA)?

    1 answer

    1. One such filter set is the Zeiss Filter Set 01 (ex=359 nm, em=461 nm).

      Helpful?

  2. Can Product D9564, DAPI dilactate (DAPI-DNA), be used as a vital stain?

    1 answer

    1. As we note in the Product Information Sheet for the Dihydrochloride salt of DAPI, Product No. D9542, DAPI can be used as a vital dye to stain mature pollen grains, but we have tested it as a vital stain in other cell types.

      Helpful?

  3. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  4. Isn't the hydrochloride salt form of DAPI a more sensitive detector of DNA than Product D9564, DAPI, dilactate (DAPI-DNA)?

    1 answer

    1. The dilactate salt has as much sensitivity in detection as the dihydrochloride salt, which we also offer as Product No. D9542.

      Helpful?

Reviews

1 of 1 reviewers received a sample product or took part in a promotion

Active Filters

  1. St. Louis, MO
    • Review 1
    • Votes 0
    4 out of 5 stars.

    Good Quality

    Arrived quickly. Labeling is dimmer than expected.

    Helpful?

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