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410217

Sigma-Aldrich

2′,7′-Dichlorofluorescein

ACS reagent

Synonyme(s) :

Dichlorofluorescein; Fluorescein 27

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About This Item

Formule empirique (notation de Hill):
C20H10Cl2O5
Numéro CAS:
76-54-0
Poids moléculaire :
401.20
Numéro Beilstein :
58009
Numéro CE :
200-968-6
Numéro MDL:
MFCD00005047
Code UNSPSC :
12171500
ID de substance PubChem :
24865700
Nomenclature NACRES :
NA.47

Qualité

ACS reagent

Niveau de qualité

200

Forme

powder

Couleur

orange to red-brown, powder

Pf

280 °C (dec.) (lit.)

Solubilité

alcohol: passes test

Densité

0.790 g/cm3

λmax

509 nm

Adéquation

passes test for adsorption indicator

Application(s)

diagnostic assay manufacturing
hematology
histology

Température de stockage

room temp

Chaîne SMILES 

Oc1cc2Oc3cc(O)c(Cl)cc3C4(OC(=O)c5ccccc45)c2cc1Cl

InChI

1S/C20H10Cl2O5/c21-13-5-11-17(7-15(13)23)26-18-8-16(24)14(22)6-12(18)20(11)10-4-2-1-3-9(10)19(25)27-20/h1-8,23-24H

Clé InChI

VFNKZQNIXUFLBC-UHFFFAOYSA-N

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Catégories apparentées

Description générale

2′,7′-Dichlorofluorescein is an oxidation-sensitive fluorescent probe.

Application

2′,7′-Dichlorofluorescein has been used to measure ROS (reactive oxygen species) formation in cells. It has been used as a fluorogenic substrate to study the peroxidase activity of saliva samples and extracts obtained from submandibular glands.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

dust mask type N95 (US), Eyeshields, Gloves

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Certificats d'analyse (COA)

Lot/Batch Number
BCCL8813
BCCK3080
BCCH5169
BCCF7954
BCBG1058V

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Aleksey Rukavishnikov et al.
Analytical biochemistry, 419(1), 9-16 (2011-08-27)
Cephalosporin was used to synthesize soluble and precipitating fluorogenic β-lactam substrates that demonstrated differential catalytic hydrolysis by three different subtypes of β-lactamase: TEM-1 (class A), p99 (class C), and a Bacillus cereus enzyme sold by Genzyme (class B). The most
Janaína Kolling et al.
Cardiovascular toxicology, 11(1), 67-73 (2010-11-16)
Hyperhomocysteinemia is a risk factor for cardiovascular disease, stroke, and thrombosis; however, the mechanisms by which homocysteine triggers these dysfunctions are not fully understood. In the present study, we investigated the effect of chronic hyperhomocysteinemia on some parameters of oxidative
Olga N Pakhomova et al.
Archives of biochemistry and biophysics, 527(1), 55-64 (2012-08-23)
Nanosecond pulsed electric field (nsPEF) is a novel modality for permeabilization of membranous structures and intracellular delivery of xenobiotics. We hypothesized that oxidative effects of nsPEF could be a separate primary mechanism responsible for bioeffects. ROS production in cultured cells
L Foucaud et al.
Toxicology in vitro : an international journal published in association with BIBRA, 24(6), 1512-1520 (2010-07-20)
The aim of this study was to investigate whether carbon black (CB) nanoparticles might induce toxicity to monocytic cells in vitro via an oxidative stress mechanism involving formation of the lipid peroxidation product 4-hydroxynonenal (4-HNE) and the subsequent role of
Andréa G K Ferreira et al.
Journal of cellular biochemistry, 113(1), 174-183 (2011-09-02)
The present study investigated the effects of chronic hyperprolinemia on oxidative and metabolic status in liver and serum of rats. Wistar rats received daily subcutaneous injections of proline from their 6th to 28th day of life. Twelve hours after the
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