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206865

Sigma-Aldrich

Primuline

Dye content 50 %

Synonyme(s) :

Direct Yellow 59, Primuline Yellow

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About This Item

Formule empirique (notation de Hill):
C21H14N3NaO3S3
Numéro CAS:
8064-60-6
Poids moléculaire :
475.54
Numéro C.I. (Colour Index):
49000
Numéro MDL:
MFCD00005798
Code UNSPSC :
12171500
ID de substance PubChem :
24852417
Nomenclature NACRES :
NA.47

Forme

powder or crystals

Niveau de qualité

100

Composition

Dye content, 50%

Pf

>300 °C

λmax

229 nm (2nd)
340-355 nm

Application(s)

diagnostic assay manufacturing
hematology
histology

Température de stockage

room temp

Chaîne SMILES 

[Na+].Cc1ccc2nc(sc2c1S([O-])(=O)=O)-c3ccc4nc(sc4c3)-c5ccc(N)cc5

InChI

1S/C21H15N3O3S3.Na/c1-11-2-8-16-18(19(11)30(25,26)27)29-21(24-16)13-5-9-15-17(10-13)28-20(23-15)12-3-6-14(22)7-4-12;/h2-10H,22H2,1H3,(H,25,26,27);/q;+1/p-1

Clé InChI

RSRNHSYYBLEMOI-UHFFFAOYSA-M

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Application

Primuline has been used as a reagent in the preparation of lipids for chloroplast lipid membrane. It has been used as a dye in preparative thin-layer chromatography (TLC).

Actions biochimiques/physiologiques

Primuline, also known as direct yellow 59 or primuline yellow, is considered to be a more sensitive and non-destructive dye for lipids. It is widely used as a fluorescent dye in preparative thin-layer chromatography (TLC). Primuline is suitable for vital staining. It is used as a fluorochrome in plant tissues. Primuline is also used to stain pollen grains and lignified cell walls. In addition, it also helps in segregating intact and broken starch grains. At pH 8, primuline acts as a fluorochrome for mast cells.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

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Certificats d'analyse (COA)

Lot/Batch Number
SHBR3189
SHBP1887
SHBM2490
MKCH8128
MKBT2168V

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J I Morrell et al.
Science (New York, N.Y.), 217(4566), 1273-1276 (1982-09-24)
A method combining steroid autoradiography and fluorescent dye retrograde neuroanatomical tracing has been devised. This method makes it possible to demonstrate that some estrogen-concentrating cells in the ventrolateral subdivision of the ventromedial nucleus of the rat hypothalamus are neurons that
[Dopamine-containing neurons retrogradely labeled with primuline--a source of descending supraspinal fibers in the rat brain].
F N Serkov et al.
Doklady Akademii nauk SSSR, 292(6), 1506-1509 (1987-01-01)
T Taki et al.
Analytical biochemistry, 223(2), 232-238 (1994-12-01)
A new and simple method for purifying glycosphingolipids and phospholipids by using "TLC blotting" was established. Glycosphingolipids separated by two-dimensional thin-layer chromatography (TLC) were made visible with primuline reagent, and then bands were marked with a drawing colored pencil. The
SEQUENTIAL SYNTHESIS AND METHYLATION OF
PHOSPHATIDYLETHANOLAMINE PROMOTES LIPID DROPLET BIOSYNTHESIS
AND STABILITY IN TISSUE CULTURE AND IN VIVO.
Horl G
The Journal of Biological Chemistry (2011)
J L Labandeira Garcia et al.
Journal of anatomy, 137 (Pt 2), 247-261 (1983-09-01)
This paper describes a qualitative and quantitative investigation into the location of the motoneurons innervating the extra-ocular muscles of the rat. Injections of horseradish peroxidase, bisbenzimide, propidium iodide and DAPI-primuline were made either in one or simultaneously in two muscles.
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