Accéder au contenu
Merck
Toutes les photos(1)

Principaux documents

SCT210

Sigma-Aldrich

BioTracker FerroOrange Live Cell Dye

Live Cell imaging probe that specifically detects labile ferrous (Fe2+) ions in live cells.

Synonyme(s) :

senescent cells IHC kit

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(1)

About This Item

Code UNSPSC :
12352207
Nomenclature NACRES :
NA.21
Le tarif et la disponibilité ne sont pas disponibles actuellement.

Forme

lyophilized

Application(s)

cell analysis

Méthode de détection

fluorometric

Description générale

Iron, the most abundant transition metal in our bodies, is involved in a number of biologically important processes such as respiration, oxygen transport, and energy production in collaboration with oxygen. In living cells, iron exists mainly as ferrous (Fe2+) and ferric (Fe3+) ionic forms. As a major contributor to oxidative damage of cells, Fe2+ is implicated in serious diseases such as cancers and neurodegenerative disorders, because of its ability to produce harmful reactive oxygen species via contact with oxygen, superoxide, and hydrogen peroxide (H2O2).BioTracker FerroOrange Live Cell Dye is an orange fluorescent probe that specifically detects labile iron (II) ions (Fe2+) only. The intensity of fluorescence does not increase in the presence of iron (III) ions (Fe3+) or bivalent metal ions other than iron. It also does not react to chelated iron in ferritin and other substances. FerroOrange is suitable for live-cell imaging because it is highly cell-permeable and has low cell toxicity.Spectral Properties:Absorbance (max): 542 nmEmission (max): 572 nmResearch Category:Live Cell Dye

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Questions

1–4 of 4 Questions  

  1. Can this kit be used for suspension cells?

    1 answer

    1. Yes, this product is suitable for use with suspension cells.

      Helpful?

  2. Is it possible to store SCT210 after reconstitution?

    1 answer

    1. The available datasheet for SCT210 provides guidance in the Reagent Preparation section, stating, "Use neutral buffer or culture medium for dilution of the DMSO solution and use immediately upon dilution. The dye could be oxidized in acidic solutions." However, there are no recommendations or data suggesting that the solutions are stable when stored. It's important to note that the package is shipped in single-use vials.

      Helpful?

  3. Is it possible to detect SCT210 FerroOrange by flow cytometry?

    1 answer

    1. The website indicates an excitation wavelength of 542 nm and an emission wavelength of 572 nm for the SCT210 FerroOrange. The product is claimed to be suitable for live cell imaging, which typically implies the use of a confocal microscope. However, given that this is a fluorescent dye, it seems reasonable to consider that the staining could be assessed using a flow cytometer. There is currently no available data documenting its use on a flow cytometer. To use it on a flow cytometer, the cells would need to be in a single cell suspension. Additionally, it is expected that the cells could be visualized using a fluorescent microscope.

      Helpful?

  4. What cells can be used as a positive control for SCT210 (BioTracker™ FerroOrange Live Cell Dye)?

    1 answer

    1. The HepG2 cell line is recommended as a positive control for SCT210 (BioTracker™ FerroOrange Live Cell Dye).

      Helpful?

Reviews

No rating value

Active Filters

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique