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SCM075

Sigma-Aldrich

Inhibiteur ROCK (Y-27632)

The ROCK Inhibitor (Y-27632) is available in a 5 mg format & has been optimized & validated for cell culture & Neuroscience applications.

Synonyme(s) :

Y-27632

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About This Item

Code UNSPSC :
12352207
eCl@ss :
32160801
Nomenclature NACRES :
NA.75

Niveau de qualité

100

Forme

liquid

Poids mol.

320.26 g/mol

Technique(s)

cell culture | mammalian: suitable

Conditions d'expédition

dry ice

Description générale

L'inhibiteur ROCK (Y-27632) est un inhibiteur de Rho kinase (ROCK), notamment des protéines p160Rock (Ki = 140 nM) et Rock-II, sélectif et compétitif de l'ATP. C'est également un puissant inhibiteur de la sensibilisation calcique par agonistes de la phosphorylation de la myosine et de la contraction des muscles lisses. Un traitement d'une heure avec l'inhibiteur Y-27632 10 µM bloque l'apoptose de cellules souches embryonnaires humaines (hESC) en culture dissociée et augmente leur survie et leur efficacité de clonage sans affecter leur pluripotence1. Récemment, des études ont démontré que l'application de l'inhibiteur Y-27632 et de la formulation d'enzymes de digestion Accutase (réf. SCR005) améliore le taux de survie post-décongélation des cellules hESC individuelles, avec des méthodes de congélation lente et de décongélation rapide.

Stockage et stabilité

Avant reconstitution : se conserve pendant deux ans à -20 ºC et à l'abri de la lumière. Après reconstitution : se conserve pendant un an à -20 ºC et à l'abri de la lumière.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Malgorzata Bienkowska-Haba et al.
Journal of virology, 94(3) (2019-11-22)
It is established that the host cell transcriptomes of natural lesions, organotypic rafts, and human papillomavirus (HPV)-immortalized keratinocytes are altered in the presence of HPV genomes. However, the establishment of HPV-harboring cell lines requires selection and immortalization, which makes it
Maike Frye et al.
eLife, 9 (2020-09-09)
Endothelial integrity is vital for homeostasis and adjusted to tissue demands. Although fluid uptake by lymphatic capillaries is a critical attribute of the lymphatic vasculature, the barrier function of collecting lymphatic vessels is also important by ensuring efficient fluid drainage
Ambra Gianotti et al.
Frontiers in pharmacology, 9, 1176-1176 (2018-11-13)
Cystic fibrosis (CF) is a chronic disease caused by mutations in the CF transmembrane conductance regulator (CFTR) gene, which encodes for a channel expressed at the apical surface of epithelial tissues. Defective chloride and bicarbonate secretion, arising from CFTR mutations
Marta Słoniecka et al.
Journal of molecular medicine (Berlin, Germany), 97(10), 1477-1489 (2019-08-11)
Fibrosis is characterized by hardening, overgrowth, and development of scars in various tissues as a result of faulty reparative processes, diseases, or chronic inflammation. During the fibrotic process in the corneal stroma of the eye, the resident cells called keratocytes
Anushree C Gulvady et al.
Molecular biology of the cell, 30(11), 1298-1313 (2019-03-21)
Fibroblasts transformed by the proto-oncogene Src form individual invadopodia that can spontaneously self-organize into large matrix-degrading superstructures called rosettes. However, the mechanisms by which the invadopodia can spatiotemporally reorganize their architecture is not well understood. Here, we show that Hic-5
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