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PIHP01250

Millipore

Inserts de culture cellulaire standards Millicell®

pore size 0.4 μm, diam. 12 mm, translucent polycarbonate membrane, Tissue Culture (TC)-treated surface, size 24 wells, sterile

Synonyme(s) :

Insert perméable pour culture cellulaire, boîte de culture tissulaire, insert de culture tissulaire, inserts de culture cellulaire

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About This Item

Code UNSPSC :
41104923
eCl@ss :
32011202
Nomenclature NACRES :
NB.22
Matériaux:
polystyrene housing
translucent polycarbonate membrane
Dimension de pores:
0.4 μm
Stérilité:
γ-irradiated
sterile
Type de liaison:
Tissue Culture (TC)-treated surface
Le tarif et la disponibilité ne sont pas disponibles actuellement.

Matériaux

polystyrene housing
translucent polycarbonate membrane

Niveau de qualité

400

Stérilité

γ-irradiated
sterile

Caractéristiques

hydrophilic
 100x106 pores/cm2 (pore density)

Conditionnement

pack of 50

Fabricant/nom de marque

Millicell®

Paramètres

50 °C max. temp.

Technique(s)

cell attachment: suitable
cell culture | mammalian: suitable
cell differentiation: suitable
drug transporter assay: suitable
permeability assay: suitable

Hauteur

10.5 mm

Diamètre

12 mm

Taille

24 wells

Superficie

0.6 cm2

Volume de travail

0.6 mL

Couleur

translucent membrane

Matrice

Isopore™

Dimension de pores

0.4 μm

Type de liaison

Tissue Culture (TC)-treated surface

Conditions d'expédition

ambient

Description générale

Configuration du dispositif : plaque 24 puits, pores de 0,4μm

Grâce aux inserts Millicell®, les cellules adhérentes ou en suspension peuvent accéder aux milieux par leurs deux pôles, apical et basolatéral. La croissance, la structure et la fonction cellulaires reproduisent plus fidèlement ce qui se passe in vivo. De plus, les inserts Millicell® permettent d'étudier les deux faces de la monocouche cellulaire.

Inserts Millicell® standards :
- Favorisent une excellente croissance cellulaire et constituent une opportunité exceptionnelle pour les études de cellules

Type de membrane :
Membrane Isopore (polycarbonate)
- Pour la croissance des cellules adhérentes, sans matrice

Applications
Adhérence cellulaire, croissance cellulaire, différenciation cellulaire, immunocytochimie

Informations légales

ISOPORE is a trademark of Merck KGaA, Darmstadt, Germany
Millicell is a registered trademark of Merck KGaA, Darmstadt, Germany

Code de la classe de stockage

10-13 - German Storage Class 10 to 13

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Peiying Tian et al.
Cell biology international, 44(8), 1681-1690 (2020-04-14)
Ghrelin-O-acyltransferase (GOAT) is a membrane-bound enzyme that attaches eight-carbon octanoate to a serine residue in ghrelin and thereby acylates inactive ghrelin to produce active ghrelin. In this study, we investigated the function of GOAT in the intestinal mucosal barrier. The
Susanne Elisabeth Pors et al.
Molecular human reproduction, 26(5), 301-311 (2020-03-24)
In vitro activation of resting ovarian follicles, with the use of mechanical stress and/or pharmacological compounds, is an emerging and novel approach for infertility treatment. The aim of this study was to assess the sphingolipid, sphingosine-1-phosphate (S1P), as a potential

Articles

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16HBE14o- human bronchial epithelial cells used to model respiratory epithelium for the research of cystic fibrosis, viral pulmonary pathology (SARS-CoV), asthma, COPD, effects of smoking and air pollution. See over 5k publications.

Protocoles

Toluidine Blue Staining Protocol for Millicell® Inserts

Toluidine blue selectively stains nuclear material and acidic tissue components, aiding in histological staining for tissues rich in DNA/RNA.

Generating Organotypic Air-Liquid Interface (ALI) 3D Skin Cultures as an In-Vitro Model of the Human Epidermis

3D cell culture protocol for generating epidermal human skin tissue using primary human keratinocytes, dermal fibroblasts, and collagen-coated transwell inserts.

Microscopic Examination of Samples

This protocol covers 3 modes for the microscopic examination of cell samples.

Indirect Co-culture of Embryonic Stem Cells with Embryonic Fibroblasts

This page covers the indirect co-culture of embryonic stem cells with embryonic fibroblasts.

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Contenu apparenté

Millicell® ERS 3.0 Digital Voltohmmeter

Streamline TEER data capture and analysis with user-friendly enhancements including an intuitive touchscreen interface, standing in-well probe, and automatic data logging.

Questions

1–3 of 3 Questions  

  1. What is the height of the "feet" of the PIHP01250 Millicell Cell Culture Inserts? Meaning, how much clearance is there between the bottom of the well and the bottom of the membrane?

    1 answer

    1. The height of the feet or distance between the membrane and bottom of the well ranges from 1 - 2 mm. Please see the image below:

      Helpful?

  2. Regarding the PIHP01250 & PTHT12H48 membrane insert products: what is the thickness of the membrane? Is the bottom totally flat, i.e. if I placed it down on a flat surface, would the entire area of the membrane be in direct contact with the surface?

    1 answer

    1. The thickness of the polycarbonate and PET membranes range from 7-20 um. The standing inserts (PIHP01250) have small "feet" that contact the bottom of the wells. The hanging inserts (PTHT12H48) are suspended by 'wings' that rest on the sides of the wells. Please see the image below:

      Helpful?

  3. What is the thickness of the membrane in these inserts? We need this information to evaluate if it's possible to image through the membrane with a confocal microscope.

    1 answer

    1. The thickness specification for the PIHP01250 Millicell Cell Culture Insert, which is made of polycarbonate and has a pore size of 0.4 µm, ranges between 9-11 microns.

      Helpful?

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