MABN720

Anti-TBX1 Antibody, clone 7E12.1

culture supernatant, clone 7E12.1, from mouse

• Synonyme(s) : T-box transcription factor TBX1, T-box protein 1, Testis-specific T-box protein
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.43

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: culture supernatant
• Type de produit anticorps: primary antibodies
• Clone: 7E12.1, monoclonal
• Espèces réactives: human, mouse
• Technique(s): western blot: suitable
• Isotype: IgG2aκ
• Numéro d'accès NCBI: NP_542377
• Numéro d'accès UniProt: O43435
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... TBX1(6899)

Description

Description générale

T-box transcription factor TBX1 (UniProt O43435; also known as T-box protein 1, Testis-specific T-box protein) is encoded by the TBX1 (also known as CTHM, DGS, TBX1C, VCFS) gene (Gene ID 6899) in human. TBX1 blongs to the T-box family of transcription factors that play important roles in the formation of tissues and organs during embryonic development. TBX1 is required for the second heart field (SHF) development for outflow tract development by regulating proliferation and differentiation of SHF cells. TBX1 is also shown to negatively regulate thymic epithelial cell (TEC) growth and differentiation in mice, extinction of Tbx1 gene expression in the third pharyngeal pouch (3rd pp) endoderm is a prerequisite for thymus organogenesis. Human TBX mutations cause DiGeorge syndrome, characterized by parathyroid hypoplasia, thymic hypoplasia, defects in the outflow tract of the heart, and craniofacial anomalies.

Spécificité

Clone 7E12.1 was raised against a recombinant fragment derived from TBX1 spliced isoform A (Uniprot O43435-1), cross-reactivity toward spliced isoform B and isoform C (TBX1C) is possible, but has not been determined.

Immunogène

Epitope: C-terminal region.

GST-tagged recombinant human TBX1 C-terminal fragment.

Application

Anti-TBX1 Antibody, clone 7E12.1 is an antibody against TBX1 for use in Western Blotting.

Research Category
Neuroscience

Research Sub Category
Transcription Factors

Western Blotting Analysis: A 1:500 dilution from a representative lot detected TBX1 in 10 µg of mouse Neuro-2a and human SH-SY5Y neuroblastoma cell lysates.

Qualité

Evaluated by Western Blotting in HUVEC lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected TBX1 in 10 µg of HUVEC lysate.

Description de la cible

~48 kDa observed. 43.13 kDa (isoform A), 40.35 kDa (isoform B), and 52.67 kDa (isoform C; TBX1C) calculated. Uncharacterized band(s) may appear in some lysates.

Liaison

Replaces: AB15624

Forme physique

Mouse monoclonal IgG2aκ hybridoma culture supernatant without preservatives.

Unpurified

Stockage et stabilité

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2