MABN14
Anti-phospho TDP-43 (Ser409/Ser410) Antibody, clone 1D3
clone 1D3, from rat
Synonyme(s) :
TAR DNA-binding protein 43, TDP-43, TDP43, TAR DNA binding protein-43, Phosphorylated TDP
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
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![Anti-phospho-TDP-43 [pSer409] antibody produced in rabbit ~1.0 mg/mL, affinity isolated antibody](/deepweb/assets/sigmaaldrich/product/images/407/478/bcd6d544-d187-42ed-8c1e-98bf709b4da7/640/bcd6d544-d187-42ed-8c1e-98bf709b4da7.jpg)
Source biologique
rat
Niveau de qualité
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
1D3, monoclonal
Espèces réactives
human
Technique(s)
ELISA: suitable
immunohistochemistry: suitable
western blot: suitable
Isotype
IgG2aκ
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
phosphorylation (pSer409/pSer410)
Informations sur le gène
human ... TARDBP(23435)
Description générale
TAR DNA-binding protein 43 (TDP-43) belongs to the hnRNP protein family and plays an important role in transcription, pre-mRNA splicing, mRNA stability and mRNA transport. It is involved in splicing of the apolipoprotein A-II and cystic fibrosis transmembrane gene. This protein is highly expressed in the pancreas, placenta, lung, genital tract and spleen. Mutations in TDP-43 have been associated with amyotrophic lateral sclerosis, frontotemporal dementia, Parkinson′s disease and Alzheimer′s disease.
Spécificité
This antibody recognizes TDP-43 when phosphorylated at Ser409/Ser410.
Immunogène
Epitope: Ser409/Ser410
Ovalbumin-conjugated linear peptide corresponding to human TDP-43 phosphorylated at Ser409/Ser410.
Application
Anti-phospho TDP-43 (Ser409/Ser410) Antibody, clone 1D3 is an antibody against phospho TDP-43 (Ser409/Ser410) for use in WB, ELISA, IH.
Immunohistochemistry Analysis: A previous lot was used by an independent laboratory in hippocampus from FTLD-U brain tissue. (Neumann, M., et al. (2009). Acta Neuropathol. 117:137–149.)
ELISA Analysis: A previous lot was used by an independent laboratory in ELISA. (Neumann, M., et al. (2009). Acta Neuropathol. 117:137–149.)
ELISA Analysis: A previous lot was used by an independent laboratory in ELISA. (Neumann, M., et al. (2009). Acta Neuropathol. 117:137–149.)
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Neurodegenerative Diseases
Qualité
Evaluated by Western Blot in human placenta cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected phosphorylated TDP-43 on 10 µg of human placenta cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected phosphorylated TDP-43 on 10 µg of human placenta cell lysate.
Description de la cible
50/25 kDa Observed
1) The 25-kDa C-terminal fragment is more prone to phosphorylation at Ser409/Ser410 than full-length TDP-43. (Zhang, Y.J., et al. (2009). PNAS. 106:7607-7612.)
2) 50-52 kDa observed MW . (Buratti, E., et al. (2001). The EMBO Journal 20:1774-1784.)
1) The 25-kDa C-terminal fragment is more prone to phosphorylation at Ser409/Ser410 than full-length TDP-43. (Zhang, Y.J., et al. (2009). PNAS. 106:7607-7612.)
2) 50-52 kDa observed MW . (Buratti, E., et al. (2001). The EMBO Journal 20:1774-1784.)
Forme physique
Format: Purified
Protein G Purified
Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Stockage et stabilité
Stable for 1 year at 2-8°C from date of receipt.
Remarque sur l'analyse
Control
Human placenta cell lysate
Human placenta cell lysate
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
10-13 - German Storage Class 10 to 13
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Patrick J Bosque et al.
PloS one, 8(5), e62301-e62301 (2013-05-25)
The transactive response DNA-binding protein (TDP-43) is a major component of the abnormal intracellular inclusions that occur in two common neurodegenerative diseases of humans: (1) a subtype of frontotemporal lobar degeneration and (2) amyotrophic lateral sclerosis. Genetics, experiments in cultured
Zhi Huang et al.
Nature communications, 14(1), 2747-2747 (2023-05-13)
Resilience to Alzheimer's disease is an uncommon combination of high disease burden without dementia that offers valuable insights into limiting clinical impact. Here we assessed 43 research participants meeting stringent criteria, 11 healthy controls, 12 resilience to Alzheimer's disease and
Pol Andrés-Benito et al.
Journal of neuropathology and experimental neurology, 78(5), 416-425 (2019-04-03)
Dyneins are major components of microtubules. Dynein assembly is modulated by a heterogeneous group of dynein axonemal assembly factors (DNAAFs). The present study analyzes dynein axonemal assembly factor 1 (DNAAF1) and leucine-rich repeat-containing protein 50 (LRRC50), the corresponding encoded protein
Jacob R Mann et al.
Neuron, 102(2), 321-338 (2019-03-04)
TDP-43 proteinopathy is a pathological hallmark of amyotrophic lateral sclerosis and frontotemporal dementia where cytoplasmic TDP-43 inclusions are observed within degenerating regions of patient postmortem tissue. The mechanism by which TDP-43 aggregates has remained elusive due to technological limitations, which
Akila Weerasekera et al.
NeuroImage. Clinical, 27, 102327-102327 (2020-07-13)
Currently TAR DNA binding protein 43 (TDP-43) pathology, underlying Amyotrophic Lateral Sclerosis (ALS), remains poorly understood which hinders both clinical diagnosis and drug discovery efforts. To better comprehend the disease pathophysiology, positron emission tomography (PET) and multi-parametric magnetic resonance imaging
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