MABF29

Anti-IL-16 Antibody, clone 14.1

clone 14.1, from mouse

• Synonyme(s) : interleukin 16 (lymphocyte chemoattractant factor), lymphocyte chemoattractant factor, pro-interleukin-16, neuronal interleukin 16, prointerleukin 16
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: 14.1, monoclonal
• Espèces réactives: human
• Technique(s): flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; neutralization: suitable; western blot: suitable
• Numéro d'accès NCBI: NP_001165599
• Numéro d'accès UniProt: Q14005
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... IL16(3603)

Description

Description générale

Originally known as lymphocyte chemoattractant factor (LCF), Interleukin-16 (IL-16) is a product of human peripheral mononuclear cells when antigen-stimulated or mitogen-stimulated. It is also produced by dendritic cells, epithelial cells, eosinophils, fibroblasts and mast cells. The pro-form of IL-16 is a 631 amino acid protein that is cleaved by caspase 3 in activated CD4+ T cells where it is then secreted and is accessible for binding CD4 receptors. IL-16 regulates and induces migratory response in CD4+ lymphocytes, eosinophils, and monocytes. It is a soluble ligand for CD4 and functions to prime responsiveness to IL-2 and IL-15 in CD4+ T-cells. Although Isoform 1’s function is thought to be as an anchor to membrane ion channels, Isoform 3 is more widely researched and characterized as component of T-cell cycle progression.

Immunogène

Epitope: Unknown

GST-tagged recombinant protein corresponding to the C-terminal region of secreted human IL-16.

Application

Detect IL-16 using this Anti-IL-16 Antibody, clone 14.1 validated for use in WB, NEUT, IH, IC, FC.

Immunohistochemistry Analysis: A previous lot was used by an independent laboratory in IH to detect IL-16 in naive kidney tissue (Wang, S., et al., 2008).

Immunocytochemistry Analysis: A previous lot was used by an independent laboratory in IC to detect IL-16 in spinal cord cells (Skundric, D., et al., 2005).

Flow Cytometry Analysis: A previous lot was used by an independent laboratory in FC to detect IL-16 in human PBMCs (McFadden, C., et al., 2007).

Neutralizing Activity Assay: 1–10 µg/mL of a previous lot was used by an independent laboratory at concentrations sufficient to neutralize bioactivity of IL-16 (Morgan, R., et al., 2007).

Research Category
Inflammation & Immunology

Research Sub Category
Cytokines & Cytokine Receptors

Qualité

Evaluated by Western Blot in human serum.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected IL-16 in 10 µg of human serum.

Description de la cible

~20 kDa observed.
Monomers of IL-16 migrate on SDS-PAGE in the range of 17–20 kDa (Zhang, Y., et al., 1998). Active IL-16 (clone 14.1) is detected at ~17 kDa in WB (Skundric, D., et al., 2005).

Forme physique

Format: Purified

Protein G Purified

Purified mouse monoclonal IgGκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
Human serum

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable