Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. ′ The assay is based on spectophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate DEVD-pNA. The free pNA can be quantified using a spectrophotometer or a microtiter plate reader at 405 nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-3 activity.
Application
&
Domaine de recherche Apoptose et cancer
Composants
5X Cell Lysis Buffer (Part No. 90065):
μμ
μ
Stockage et stabilité
Conserver les composants du kit à -20 °C jusqu'à leur date de péremption.
Special Precautions:
· After thawing reagents, use immediately or aliquot and freeze at -20oC for longer storage. Éviter les congélation/décongélation répétées.
· Maintain these reagents in amber or covered containers.
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Code de la classe de stockage
10 - Combustible liquids
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