ABD135

Anti-NANOS3 Antibody

from rabbit, purified by affinity chromatography

• Synonyme(s) : Nanos homolog 3, NOS-3, NOS3
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: human
• Technique(s): immunofluorescence: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• Numéro d'accès UniProt: P60323
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... NANOS3(342977)

Description

Description générale

Nanos homolog 3 (Nanos3), also known as NOS-3 or NOS3, belongs to the Nanos family. Nanos is a zinc-finger containing, RNA-binding protein that has been implicated in germ cell development in both invertebrates and vertebrates. Nanos3 has been shown to play an important role in the maintenance of the undifferentiated state of germ cells regulating the spermatogonia cell cycle and inducing a prolonged transit in G1 phase. Nanos3 has also been shown to be essential in the early stage embryo protecting the migrating primordial germ cells (PGCs) from apoptosis. Additionally, it is implied to maintain the germ cell lineage by suppressing both Bax-dependent and -independent apoptotic pathways and to affect cell proliferation by repressing translation of specific mRNAs. Nanos3 is expressed primarily in ovary, testis and brain. In the ovaries, Nanos3 is expressed during multiple stages of oogenesis, including primordial, primary, secondary and antral follicles with the highest expression in the oocytes. In the testis, Nonos3 is expressed in germ cells, type A spermatogonia (SA), primary spermatocytes (S1), round spermatids (S3) and elongated spermatids.

Spécificité

This antibody recognizes the C-terminus of NANOS3.

Immunogène

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of human NANOS3.

Application

Detect Nanos homolog 3 using this rabbit polyclonal antibody, Anti-NANOS3 Antibody validated for use in western blotting, IHC (Paraffin) & Immunofluorescence.

Immunohistochemistry Analysis: 2.5 µg/mL from a representative lot detected NANOS3 in human brain tissue.

Immunofluorescence Analysis: 20 μg/mL from a representative lot detected NANOS3 in human brain cells.

Research Category
Stem Cell Research

Research Sub Category
Pluripotent & Early Differentiation

Qualité

Evaluated by Western Blotting in human brain tissue lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected NANOS3 in 15 µg of human brain tissue lysate.

Description de la cible

~21 kDa observed. Uncharacterized bands may appear in some lysate(s). Uniprot describes 2 isoforms at ~19 kDa and ~21 kDa

Forme physique

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
Human brain tissue lysate

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable