517310
Anti-P-Glycoprotein Mouse mAb (C219)
liquid, clone C219, Calbiochem®
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About This Item
Code UNSPSC :
12352203
Produits recommandés
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
purified antibody
Type de produit anticorps
primary antibodies
Clone
C219, monoclonal
Forme
liquid
Contient
≤0.1% sodium azide as preservative
Espèces réactives
rat, hamster, human, mouse
Fabricant/nom de marque
Calbiochem®
Conditions de stockage
OK to freeze
avoid repeated freeze/thaw cycles
Isotype
IgG1
Conditions d'expédition
wet ice
Température de stockage
−20°C
Informations sur le gène
human ... ABCB1(5243)
Description générale
Purified mouse monoclonal antibody. Recognizes the ~170 kDa MDR1 and MDR3 isoforms of P-glycoprotein.
Recognizes the ~170 kDa MDR1 and MDR3 isoforms of P-glycoprotein.
This Anti-P-Glycoprotein Mouse mAb (C219) is validated for use in FC, Frozen Sections, Immunoblotting, ICC, IP, Paraffin Sections for the detection of P-Glycoprotein.
Immunogène
Hamster and Human
SDS-solubilized plasma membranes of a multidrug resistant (MDR) Chinese hamster ovary (CHO) cell line and a multidrug resistant human cell line
Application
Flow Cytometry (5-10 µg/1 x 10⁶ cells)
Frozen Sections (5-10 µg/ml)
Immunoblotting (1-10 µg/ml, see application references, see comments)
Immunocytochemistry (see comments)
Immunoprecipitation (see comments)
Paraffin Sections (1-10 µg/ml)
Frozen Sections (5-10 µg/ml)
Immunoblotting (1-10 µg/ml, see application references, see comments)
Immunocytochemistry (see comments)
Immunoprecipitation (see comments)
Paraffin Sections (1-10 µg/ml)
Conditionnement
Please refer to vial label for lot-specific concentration.
Avertissement
Toxicity: Standard Handling (A)
Forme physique
In PBS, 1% BSA.
Reconstitution
Following initial thaw, aliquot and freeze (-20°C).
Remarque sur l'analyse
Positive Control
Cell lines or frozen tissue, such as liver or colon
Cell lines or frozen tissue, such as liver or colon
Autres remarques
Also recognizes ~150 kDa sister P-glycoprotein (SPGP). Frozen sections should be fixed in acetone for 10 min at -20°C. Formalin-fixed, paraffin tissue sections should be deparaffinized and rehydrated by conventional means. Boiling samples may reduce visibility of the protein by immunoblotting (see application references). This antibody is also reported to work for immunocytochemistry and immunoprecipitation. A ~200 kDa protein that migrates in the same position as myosin is also seen by immunoblotting. Variables associated with assay conditions will dictate the proper working dilutions.
Beck, W.T., et al. 1996. Cancer Res. 56, 3010.
Toth, K., et al. 1992. Am. J. Pathol. 140, 1009.
Schinkel, A.H., et al. 1991. Cancer Res. 51, 2628.
Chan, H.S.L., et al. 1988. Lab Invest. 59, 870.
Toth, K., et al. 1992. Am. J. Pathol. 140, 1009.
Schinkel, A.H., et al. 1991. Cancer Res. 51, 2628.
Chan, H.S.L., et al. 1988. Lab Invest. 59, 870.
Informations légales
ImmunoblottingLee, G., et al. 2001. J. Pharmacol. Exp. Ther.299, 204.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Linlin Su et al.
The Journal of endocrinology, 209(3), 337-351 (2011-04-08)
The blood--testis barrier (BTB) creates an immunological barrier that segregates the seminiferous epithelium into the basal and apical compartment. Thus, meiosis I/II and post-meiotic germ cell development take place in a specialized microenvironment in the apical compartment behind the BTB
Yan Chen et al.
PloS one, 10(10), e0140918-e0140918 (2015-10-21)
The objective of the present study was to investigate the role of high-mobility group box-1 (HMGB1) in the seizure-induced P-glycoprotein (P-gp) overexpression and the underlying mechanism. Kainic acid (KA)-induced mouse seizure model was used for in vivo experiments. Male C57BL/6
Linlin Su et al.
The international journal of biochemistry & cell biology, 41(12), 2578-2587 (2009-09-02)
Throughout spermatogenesis, leptotene spermatocytes traverse the blood-testis barrier (BTB) to enter the adluminal compartment of the seminiferous epithelium for continued development. At the same time, the integrity of the BTB, which is constituted by co-existing tight junctions (TJ), basal ectoplasmic
Lu Jiang et al.
Journal of experimental & clinical cancer research : CR, 38(1), 265-265 (2019-06-20)
Discovery and development of novel drugs that are capable of overcoming drug resistance in tumor cells are urgently needed clinically. In this study, we sought to explore whether ivermectin (IVM), a macrolide antiparasitic agent, could overcome the resistance of cancer
Prashant Bhattarai et al.
Journal of drug targeting, 25(5), 436-450 (2016-12-13)
Drug resistance is a common phenomenon that occurs in cancer chemotherapy. Delivery of chemotherapeutic agents as polymer pro-drug conjugates (PPDCs) pretargeted with bispecific antibodies could circumvent drug resistance in cancer cells. To demonstrate this approach to overcome drug resistance, Paclitaxel
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