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Supelco

Purospher® STAR NH2 (5 µm) HPLC Columns

L × I.D. 250 mm x 4.6 mm LiChroCART® HPLC cartridge, manuCART™ holder required for use

Synonyme(s) :

Purospher® STAR NH2 (5 µm) LiChroCART® 250-4.6

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About This Item

Code UNSPSC :
41115709
Nomenclature NACRES :
SB.52

product name

Purospher® STAR NH2 (5 µm), L × I.D. 250 mm x 4.6 mm LiChroCART® HPLC cartridge, manuCART™ holder required for use

Matériaux

stainless steel column

Niveau de qualité

100

Agence

suitable for USP L8

Gamme de produits

Purospher®

Caractéristiques

endcapped: no

Conditionnement

1 ea of

Ampleur du marquage

3.5% Carbon loading

Paramètres

250 bar pressure
60 °C max. temp.

Technique(s)

HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (Low - Medium)

Colonne, L × D.I.

25 cm × 4.6 mm

Superficie

330 m2/g

Matrice

fully porous particle

Groupe de la matrice active

NH2 bonding phase

Taille des particules

5 μm

Dimension de pores

120 Å pore size

operating pH range

2-7.5

Technique de séparation

hydrophilic interaction (HILIC)
normal phase
reversed phase

Température de stockage

no temp limit

Catégories apparentées

Description générale

Purospher® STAR NH2, 5 µm HPLC columns are widely used for carbohydrate analysis, with a typical mobile phase consisting of acetonitrile and water. In terms of their polarity, these columns lie between bare silica and reversed-phase silica. Hence, Purospher® STAR NH2 can also be used as an ion-exchanger. In acidic solutions, the NH2-groups are protonated (-NH3 +X-) and therefore display the characteristics of a weak anion exchanger. Medium polarity Purospher® STAR NH2 columns possess hydrophilic as well as hydrophobic properties, thus can be used under both reversed-phase (HILIC) and normal-phase conditions. However, retention is weaker than on silica or RP-supports. Purospher® STAR NH2 columns are based on ultra-pure fully porous silica particles (Type B). Purospher® STAR NH2, 5 µm LiChroCART® 250-4.6 is a cartridge column which requires a manuCART™ cartridge column holder part number 1.51486.0001.The recommended guard column is Purospher® STAR NH2 (5 µm) LiChroCART® 4-4 part number 1.50267.0001. LiChroCART® cartridge columns mounted with a manuCART™ cartridge column holder is hand-tight only and have therefore a maximum pressure stability of 250 bar. For higher pressure stability requirements Purospher® STAR NH2, 5 µm Hibar® RT 250-4.6 HPLC columns are available part number 1.51913.0001.The column dimension of 250 mm length x 4.6 mm I.D. is the optimal choice for the HPLC separation of complex.Learn more about Purospher® STAR HPLC and UHPLC ColumnsLearn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection GuideBrochure: A Practical Guide to HPLC - Method Development Guide

Application

Intended to be used in the separation of alcohols,carboxylic acids, fat soluble vitamins, nucleotides, steroids, sugars, sugar alcohols, sweeteners, and water soluble vitamins.

Caractéristiques et avantages

  • Ultra-pure silica (99.999%)
  • Low silanol activity
  • Reproducible results from run-to-run and batch-to-batch

Remarque sur l'analyse

Pressure: ≤ 60 bar
Theoretical Plates (N/m) (2-Nitrotoluene): ≥ 50000
Symmetry (2-Nitrotoluene): 0.8 - 1.4
Capacity factor (2-Nitrotoluene): ≤ 3.0

Informations légales

Hibar is a registered trademark of Merck KGaA, Darmstadt, Germany
LICHROCART is a registered trademark of Merck KGaA, Darmstadt, Germany
MANUCART is a trademark of Merck KGaA, Darmstadt, Germany
Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany

Code de la classe de stockage

11 - Combustible Solids

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