14-779-M

MKP5, active

• Code UNSPSC : 12352204
• eCl@ss : 32160405
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: human
• Niveau de qualité: 100
• Poids mol.: M_w 20.6 kDa
• Fabricant/nom de marque: Upstate^®
• Technique(s): activity assay: suitable (phosphatase)
• Numéro d'accès NCBI: NM_007207

Description

Description générale

Dual specificity protein phosphatases inactivate their target kinases by dephosphorylating both the
phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the MAPK superfamily
(MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. Different members ofthis family of dual specificity phosphatases show distinct substrate specificities for MAPKs, different tissue
distribution and subcellular localization, and different modes of inducibility of their expression by extracellular
stimuli. This gene product binds to and inactivates p38 and SAPK/JNK, but not MAPK/ERK. Its subcellular localization
is unique; it is evenly distributed in both the cytoplasm and the nucleus. This gene is widely expressed in various
tissues and organs, and its expression is elevated by stress stimuli. Three transcript variants encoding two different
isoforms have been found for this gene.

N-terminal 6His-tagged, recombinant, human MKP5, amino acids 320-end, expressed in E. coli.

Conditionnement

Also available in
250μg
size as catalog #
14-779M
and in
bulk
as catalog #
14-779-K
.

Qualité

Routinely evaluated by the release of phosphate from the substrate 6,8-difluoro-4-methylumbelliferyl phosphate (DiFMUP)

Forme physique

Protein in 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.

Format: Purified using Ni2+/NTA agarose.

Stockage et stabilité

6 months at -70°C

Autres remarques

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Informations légales

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

Informations sur la sécurité

• Pictogrammes: GHS07
• Mention d'avertissement: Warning
• Mentions de danger: H317
• Conseils de prudence: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Classification des risques: Skin Sens. 1
• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable