05-1587

Anti-FOXO3a/FKHRL1 Antibody, clone 15F7.2

clone 15F7.2, from mouse

• Synonyme(s) : Forkhead box protein O3, AF6q21 protein, Forkhead in rhabdomyosarcoma-like 1
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: 15F7.2, monoclonal
• Espèces réactives: human, rat
• Technique(s): immunohistochemistry: suitable; western blot: suitable
• Isotype: IgG1κ
• Numéro d'accès NCBI: NP_001446
• Numéro d'accès UniProt: O43524
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... FOXO3(2309)

Description

Description générale

Forkhead box protein O3 (FOXO3a), or FKHRL1, is a ubiquitous transcription factor that triggers the expression of pro-apoptotic genes under conditions of oxidative stress. FOXO3a is regulated by the Akt kinase which phosphorylates FOXO3a on serine 253, 315, and on threonine 32 residues. Modified FOXO3a is recognized by the 14-3-3 proteins which export FOXO3a to the cytoplasm, and consequently terminates its transcriptional activity. FOXO3a may also be regulated by lysine methylation in neuronal cells.

Immunogène

GST-tagged recombinant protein corresponding to human FOX03a.

Application

Anti-FOXO3a/FKHRL1 Antibody, clone 15F7.2 is a Mouse Monoclonal Antibody for detection of FOXO3a/FKHRL1 also known as Forkhead box protein O3, AF6q21 protein, Forkhead in rhabdomyosarcoma-like 1 & has been validated in WB & IHC.

Immunohistochemistry Analysis: A 1:300 dilution from a representative lot detected FOXO3a/FKHRL1 in human thyroid gland tissue and human breast cancer tissue.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Apoptosis - Additional

Qualité

Evaluated by Western Blot in rat liver tissue lysate.

Western Blot Analysis: 0.2 µg/mL of this antibody detected FOXO3a/FKHRL1 in 10 mg of rat liver tissue lysate.

Description de la cible

~71 kDa observed. An uncharacterized band may be observed at ~28 kDa in some tissue lysates.

Liaison

Replaces: 04-1007

Forme physique

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
Rat liver tissue lysate.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable