05-1470
Anti-Raptor Antibody, clone 1H6.2
clone 1H6.2, from mouse
Synonyme(s) :
P150 target of rapamycin (TOR)-scaffold protein, p150 target of rapamycin (TOR)-scaffold protein containing WD-repeats, regulatory associated protein of mTOR
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
1H6.2, monoclonal
Espèces réactives
mouse, human
Technique(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Isotype
IgG1κ
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... RPTOR(57521)
mouse ... Rptor(74370)
Description générale
Raptor is a subunit of mTORC1 which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Within mTORC1, Raptor positively regulates mTORC1 activity and functions as a scaffold for recruiting mTORC1 substrates.
Spécificité
This antibody recognizes Raptor.
Immunogène
Epitope: Unknown
Recombinant protein corresponding to human Raptor.
Application
Anti-Raptor Antibody, clone 1H6.2 detects level of Raptor & has been published & validated for use in WB, IP & IC.
Immunocytochemistry Analysis: A 1:500 dilution from a previous lot detected Raptor in NIH/3T3 cells.
Immunoprecipitation Analysis: 20 µg from a previous lot immunoprecipitated Raptor in HEK293 cell lysate.
Immunoprecipitation Analysis: 20 µg from a previous lot immunoprecipitated Raptor in HEK293 cell lysate.
Research Category
Signaling
Signaling
Research Sub Category
PI3K, Akt, & mTOR Signaling
PI3K, Akt, & mTOR Signaling
Qualité
Evaluated by Western Blot in HEK293 cell lysate.
Western Blot Analysis: 1 µg/ml of this antibody detected Raptor on 10 µg of HEK 293 cell lysate.
Western Blot Analysis: 1 µg/ml of this antibody detected Raptor on 10 µg of HEK 293 cell lysate.
Description de la cible
~ 150 kDa
Forme physique
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Stockage et stabilité
Stable for 1 year at 2-8°C from date of receipt.
Remarque sur l'analyse
Control
HEK 293 cell lysate
HEK 293 cell lysate
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Sungki Hong et al.
Methods in molecular biology (Clifton, N.J.), 821, 29-44 (2011-11-30)
mTOR, an evolutionarily conserved Ser/Thr protein kinase, belongs to the PI3K-related kinase family, which also includes DNA-PKcs, ATM, and ATR. Although other PI3K-related kinase family members have been shown to secure genomic integrity by sensing DNA damage and related stresses
Abdessattar Khlaifia et al.
Molecular brain, 15(1), 56-56 (2022-06-18)
Hippocampal CA1 parvalbumin-expressing interneurons (PV INs) play a central role in controlling principal cell activity and orchestrating network oscillations. PV INs receive excitatory inputs from CA3 Schaffer collaterals and local CA1 pyramidal cells, and they provide perisomatic inhibition. Schaffer collateral
Julia Dyachok et al.
The Journal of biological chemistry, 291(43), 22414-22426 (2016-09-03)
The mechanistic target of rapamycin complex 1 (mTORC1) coordinates cell growth with its nutritional, hormonal, energy, and stress status. Amino acids are critical regulators of mTORC1 that permit other inputs to mTORC1 activity. However, the roles of individual amino acids
Radhika Chadha et al.
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology, 45(6), 1059-1067 (2020-01-18)
Abnormal neurotransmission is central to schizophrenia (SZ). Alterations across multiple neurotransmitter systems in SZ suggest that this illness may be associated with dysregulation of core intracellular processes such as signaling pathways that underlie the regulation and integration of these systems.
Milagros Suarez et al.
Oncotarget, 12(10), 955-966 (2021-05-21)
The MAPK-interacting kinases 1 and 2 (MNK1/2) have generated increasing interest as therapeutic targets for acute myeloid leukemia (AML). We evaluated the therapeutic potential of the highly-selective MNK1/2 inhibitor Tomivosertib on AML cells. Tomivosertib was highly effective at blocking eIF4E
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