Gold nanoparticles, 40 nm, NHS ester functionalized, conjugation kit is a comprehensive kit that enables easy, one-step conjugation of your amine-containing ligands to 40 nm gold nanoparticles. The kit includes pre-made mixtures of lyophilized (freeze-dried) NHS-activated gold nanoparticles, along with all the necessary materials for three independent conjugation reactions. The kit components comprise: •3 vials of 40 nm NHS-Activated Gold Nanoparticles (lyophilized) •Protein Resuspension Buffer - 1.5ml •Reaction Buffer - 1.5ml •Quencher Solution - 1.5ml •Reaction protocol The gold nanoparticles in this kit are supplied as a lyophilized (freeze-dried) mixture. The conjugation reaction is initiated by simply reconstituting the freeze-dried nanoparticles with the protein of interest, which attaches to the nanoparticle surface via lysine residues. The gold conjugation procedure has a quick hands-on time of around 10 minutes, and the conjugate is fully ready for use within 3 hr. Researchers only need to pipette the biomolecule into a vial containing the nanoparticles, quench the reaction, and perform product washing through microcentrifugation. The provided protocol is optimized for highly efficient covalent conjugation, resulting in stable gold nanoparticle conjugates. Optional protocol steps involve additional materials, such as 10% aqueous BSA (126615) and Tris saline resuspension buffer (PPB002), which are sold separately.
Application
This gold conjugation kit streamlines the process of conjugating your proteins or biomolecules to 40 nm gold nanoparticles. Gold conjugation helps researchers to visualize and assay a specific protein or antibody of interest. Gold conjugates are useful in a wide range of applications like lateral flow testing, immunoblotting, immuno-electron microscopy, immunohistochemical staining, and other bio-assays. Different sizes of gold nanoparticles perform better in specific applications. For lateral flow and flow cytometry, we recommend using gold nanoparticles with diameters between 20 nm and 100 nm. For immunoblotting or immuno-electron microscopy, the optimal range is 5 nm to 40 nm. For bioassays utilizing dynamic light scattering, gold nanoparticles with diameters ranging from 60 nm to 100 nm are suggested.
Caractéristiques et avantages
•The resulting covalent conjugates offer greater stability compared to those prepared using passive adsorption methods. •Fast and convenient conjugation reaction with no need for pre-activation. •A spacer between the gold nanoparticle surface and the conjugated ligand minimizes the impact on the tertiary protein structure. This reduces the likelihood of poor performing conjugates, a common issue encountered with conjugates prepared by passive adsorption. •Gold surface coating is precisely engineered to minimize non-specific protein binding in biological assays.
Informations légales
Product of CytoDiagnostics, Inc.
Code de la classe de stockage
10 - Combustible liquids
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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Journal of pharmaceutical and biomedical analysis, 181, 113101-113101 (2020-01-27)
We evaluated the use of gold nanoparticles (AuNPs) platform in a homogenous assay for a potency measurement of a therapeutic monoclonal antibody (mAb). The recombinant human ligand protein to the therapeutic mAb was immobilized on AuNPs via functionalized self-assembled monolayers.
Facile modification of ITO electrodes with functional materials through catalytic grafting of N-hydroxysuccinimidyl-ester-functionalized methallylsilane
Development and clinical application of a rapid IgM-IgG combined antibody test for SARS-CoV-2 infection diagnosis
Li Z, et al.
Journal of Medical Virology, 78, 396-403 (2020)
Immobilization strategy for enhancing sensitivity of immunosensors: L-Asparagine-AuNPs as a promising alternative of EDC-NHS activated citrate-AuNPs for antibody immobilization
Raghav R and Srivastava S
Biosensors And Bioelectronics, 78, 396-403 (2016)
Recent advances in nanoparticle-based lateral flow immunoassay as a point-of-care diagnostic tool for infectious agents and diseases
i prodotti 900483 e 900477 si potrebbe avere una scheda tecnica al riguardo ?
Questi prodotti comprendono 1kit, 3 kit o 10 kit? Perchè non lo scrivete.
1 answer
Technical Support
·2 years ago
Thank you for calling this to our attention. Additional information will be added to the product page shortly. Each product includes NHS-Activated Gold Nanoparticles, a protein resuspension buffer, a reaction buffer, and a quencher solution. As mentioned on the website, these products are of CytoDiagnostics, Inc.
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