Platelet Derived Growth Factors (PDGF)

Jennifer Fries

Platelet-Derived Growth Factor is the principal mitogen found in mammalian serum and is released from platelets during clot formation.¹ PDGF elicits multifunctional actions with a variety of cells, including mitogenesis of mesoderm-derived cells, increased extracellular matrix synthesis, and chemotaxis and activation of neutrophils, monocytes and fibroblasts. PDGF is mitogenic for dermal and tendon fibroblasts, vascular smooth muscle cells, glial cells and chondrocytes. PDGF appears to interact with Transforming Growth Factor-1 in accelerating wound healing.² However, PDGF may also be pathogenic in arteriosclerosis and neoplasia.³ The mitogenic activities of all PDGF products are tested in culture using Swiss 3T3 cells or NR6-3T3 fibroblasts.⁴

Platelet Derived Growth Factors (PDGF)

Product Description	Source	Recombinant Host	Physical Form	Assay	Cell Type Affected	Cat. No.
Platelet-Derived Endothelial Cell Growth Factor	human	Sf21 cells	lyophilized powder	The proliferative activity of human PD-ECGF is measured in culture by using human umbilical vein endothelial cells.	vascular endothelial cell	P5208-10UG
Platelet-Derived Growth Factor	from human platelets	-	lyophilized powder	The mitogenic activity is tested in culture using Swiss 3T3 cells or NR6-3T3 fibroblasts.	chrondrocyte mesenchymal cell fibroblast glial cell muscle cell	P8147-1VL
Platelet-Derived Growth Factor	from porcine platelets	-	lyophilized powder	The mitogenic activity is tested in culture using Swiss 3T3 cells or NR6-3T3 fibroblasts.	glial cell fibroblast mesenchymal cell chrondrocyte muscle cell	P8953-5UG

Platelet Derived Growth Factors - AA (PDGF-AA)

Product Description	Source	Recombinant Host	Physical Form	Assay	Cell Type Affected	Cat. No.
Platelet-Derived Growth Factor-AA	human	Escherichia coli	lyophilized powder	The biological activity is measured by the dosedependent stimulation of thymidine uptake by BALB/c 3T3 cells.	chrondrocyte glial cell fibroblast mesenchymal cell muscle cell	P3076-10UG

Platelet Derived Growth Factors - AB (PDGF-AB)

Product Description	Source	Recombinant Host	Physical Form	Assay	Cell Type Affected	Cat. No.
Platelet-Derived Growth Factor-AB	human	Escherichia coli	lyophilized powder	The biological activity is measured by its ability to stimulate 3H-thymidine incorporation in quiescent NR6R-3T3 fibroblasts.	chrondrocyte glial cell fibroblast mesenchymal cell muscle cell	P3326-10UG

Platelet Derived Growth Factors - BB (PDGF-BB)

Product Description	Source	Recombinant Host	Physical Form	Assay	Cell Type Affected	Cat. No.
Platelet-Derived Growth Factor-BB	human	Escherichia coli	lyophilized powder	The biological activity is measured by its ability to stimulate 3H-thymidine incorporation in quiescent NR6R-3T3 fibroblasts.	fibroblast glial cell muscle cell chrondrocyte mesenchymal cell	P3201-10UG
Platelet-Derived Growth Factor-BB	from rat	Escherichia coli	lyophilized powder	The biological activity is measured by its ability to stimulate 3H-thymidine incorporation in quiescent NR6R-3T3 fibroblasts.	glial cell chrondrocyte muscle cell mesenchymal cell fibroblast	P4056-50UG
Platelet-Derived Growth Factor-BB	human	Escherichia coli	lyophilized powder	The biological activity is measured by the dosedependent stimulation of thymidine uptake in BALB/c 3T3 cells.	glial cell muscle cell chrondrocyte mesenchymal cell fibroblast	P4306-10UG

References

1. Ross R, Glomset J, Kariya B, Harker L. 1974. A Platelet-Dependent Serum Factor That Stimulates the Proliferation of Arterial Smooth Muscle Cells In Vitro. Proceedings of the National Academy of Sciences. 71(4):1207-1210. https://doi.org/10.1073/pnas.71.4.1207

2. Pierce GF, Mustoe TA, Lingelbach J, Masakowski VR, Griffin GL, Senior RM, Deuel TF. 1989. Platelet-derived growth factor and transforming growth factor-beta enhance tissue repair activities by unique mechanisms.. 109(1):429-440. https://doi.org/10.1083/jcb.109.1.429

3. Ross R. 1981. George Lyman Duff Memorial Lecture. Atherosclerosis: a problem of the biology of arterial wall cells and their interactions with blood components.. Arteriosclerosis. 1(5):293-311. https://doi.org/10.1161/01.atv.1.5.293

4. Raines EW, Ross R. 1985. [58] Purification of human platelet-derived growth factor.749-773. https://doi.org/10.1016/0076-6879(85)09128-5