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An HPLC method for the determination of selected amino acids in human embryo culture medium.

Biomedical chromatography : BMC (2016-07-28)
Petra Drábková, Lenka Andrlová, Roman Kanďár
PMID27460393
ZUSAMMENFASSUNG

A method for the determination of selected amino acids in culture medium using HPLC with fluorescence detection is described. Twenty hours after intra-cytoplasmic sperm injection, one randomly selected zygote was transferred to the culture medium. After incubation (72 h after fertilization), the culture medium in which the embryo was incubated and blank medium was immediately stored at -80°C. Filtered medium samples were derivatized with ortho-phthalaldehyde (naphthalene-2,3-dicarboxaldehyde), forming highly fluorescent amino acids derivatives. Reverse-phase columns (LichroCART, Purospher STAR RP

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Marke
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53829-U
Supelco
Ascentis® Express C18, 2,7 μm HPLC-Säule, 2.7 μm particle size, L × I.D. 15 cm × 4.6 mm
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