- Mapping the lipolytic proteome of adipose tissue using fluorescent suicide inhibitors.
Mapping the lipolytic proteome of adipose tissue using fluorescent suicide inhibitors.
Methods in molecular biology (Clifton, N.J.) (2009-09-19)
Maximilian Schicher, Manfred Kollroser, Albin Hermetter
PMID19763492
ZUSAMMENFASSUNG
Lipases are responsible for the hydrolysis of acylglycerols and cholesteryl esters in animals, plants, and microorganisms. In this chapter we describe a tool for the concomitant analysis of lipases in complex proteomes. For this purpose, the target enzymes are selectively and covalently labelled with fluorescent suicide inhibitors. Stable lipid-protein complexes are formed that are separated by gel electrophoresis and identified by mass spectrometry.
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Marke
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