- In situ activation of bis-dialkylaminophosphines--a new method for synthesizing deoxyoligonucleotides on polymer supports.
In situ activation of bis-dialkylaminophosphines--a new method for synthesizing deoxyoligonucleotides on polymer supports.
Nucleic acids research (1984-05-25)
A D Barone, J Y Tang, M H Caruthers
PMID6728676
ZUSAMMENFASSUNG
Deoxynucleoside phosphoramidites can be prepared in good yield from deoxynucleosides, bis- dialkylaminophosphines , and the corresponding dialkylamine hydrotetrazolide or tetrazole as catalysts. These phosphoramidites generated in situ lead to the direct synthesis of deoxyoligonucleotides on polymer supports.
MATERIALIEN
Produktnummer
Marke
Produktbeschreibung
Sigma-Aldrich
Tetrazol -Lösung, suitable for DNA synthesis, filtered through a 1 μm filter, ~0.45 M in acetonitrile