- Cationic heme undecapeptide as stain for detecting glycosaminoglycans on cellulose acetate strips.
Cationic heme undecapeptide as stain for detecting glycosaminoglycans on cellulose acetate strips.
Analytical biochemistry (1986-05-15)
N Ghinea
PMID2424338
ZUSAMMENFASSUNG
A rapid and sensitive method has been developed for detecting glycosaminoglycans on cellulose acetate strips. The method is based on the electrostatic interactions between glycosaminoglycans and cationic heme undecapeptide. The complexes are identified by peroxidatic reaction using 3,3'-diaminobenzidine as H2 donor. The detection limit is 2.5-10 ng for sulfated glycosaminoglycans and 60 ng for hyaluronic acid. The sensitivity of this method is higher than of those using Alcian blue or toluidine blue (500 ng).