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Artificial polypeptide scaffold for protein immobilization.

Journal of the American Chemical Society (2005-07-21)
Kechun Zhang, Michael R Diehl, David A Tirrell
ZUSAMMENFASSUNG

An artificial polypeptide scaffold composed of surface anchor and protein capture domains was designed and expressed in vivo. By using a mutant E. coli phenylalanyl-tRNA synthetase, the photoreactive amino acid para-azidophenylalanine was incorporated into the surface anchor domain. Octyltrichlorosilane-treated surfaces were functionalized with this polypeptide by spin coating and photocrosslinking. The resulting protein films were shown to immobilize recombinant proteins through association of coiled coil heterodimer.

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Sigma-Aldrich
Trichlor(octyl)silan, 97%