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ImmunoPET/MR imaging allows specific detection of Aspergillus fumigatus lung infection in vivo.

Proceedings of the National Academy of Sciences of the United States of America (2016-01-21)
Anna-Maria Rolle, Mike Hasenberg, Christopher R Thornton, Djamschid Solouk-Saran, Linda Männ, Juliane Weski, Andreas Maurer, Eliane Fischer, Philipp R Spycher, Roger Schibli, Frederic Boschetti, Sabine Stegemann-Koniszewski, Dunja Bruder, Gregory W Severin, Stella E Autenrieth, Sven Krappmann, Genna Davies, Bernd J Pichler, Matthias Gunzer, Stefan Wiehr
ZUSAMMENFASSUNG

Invasive pulmonary aspergillosis (IPA) is a life-threatening lung disease caused by the fungus Aspergillus fumigatus, and is a leading cause of invasive fungal infection-related mortality and morbidity in patients with hematological malignancies and bone marrow transplants. We developed and tested a novel probe for noninvasive detection of A. fumigatus lung infection based on antibody-guided positron emission tomography and magnetic resonance (immunoPET/MR) imaging. Administration of a [(64)Cu]DOTA-labeled A. fumigatus-specific monoclonal antibody (mAb), JF5, to neutrophil-depleted A. fumigatus-infected mice allowed specific localization of lung infection when combined with PET. Optical imaging with a fluorochrome-labeled version of the mAb showed colocalization with invasive hyphae. The mAb-based newly developed PET tracer [(64)Cu]DOTA-JF5 distinguished IPA from bacterial lung infections and, in contrast to [(18)F]FDG-PET, discriminated IPA from a general increase in metabolic activity associated with lung inflammation. To our knowledge, this is the first time that antibody-guided in vivo imaging has been used for noninvasive diagnosis of a fungal lung disease (IPA) of humans, an approach with enormous potential for diagnosis of infectious diseases and with potential for clinical translation.

MATERIALIEN
Produktnummer
Marke
Produktbeschreibung

Sigma-Aldrich
Anti-Mouse Polyvalent Immunoglobulins (G,A,M)−FITC antibody produced in goat, affinity isolated antibody, buffered aqueous solution
Sigma-Aldrich
Anti-Mouse IgG3 (γ-chain specific) antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution