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S8398

Sigma-Aldrich

Shields and Sang M3 Insect Medium

With L-glutamine, without potassium bicarbonate, powder, suitable for insect cell culture

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About This Item

MDL-Nummer:
MFCD00243218
UNSPSC-Code:
12352207
NACRES:
NA.75

Form

powder

Methode(n)

cell culture | insect: suitable

Komponenten

NaHCO3: no
L-glutamine: 0.6 g/L
glucose: 10 g/L (Dextro)

Versandbedingung

ambient

Lagertemp.

2-8°C

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Allgemeine Beschreibung

Shields and Sang M3 insect medium can support the rapid growth of both primary and established cultures of Drosophila melanogaster cells. Insect tissue culture media have been formulated to mimic the main properties of the hemolymph of specific insects.

Anwendung

Shields and Sang M3 Insect Medium has been used:
  • for the dissection of Drosophila larvae to study the changes in the translational activity of larval wing imaginal disc cells
  • to incubate Drosophila bodies for surface sensing of translation (SUnSET) assay
  • to culture Drosophila cells for transfection studies

Developed for the culture of Drosophila cell lines.

Piktogramme

Exclamation mark
GHS07

Signalwort

Warning

H-Sätze

H319

Gefahreneinstufungen

Eye Irrit. 2

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 2

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Tim Hundertmark et al.
PloS one, 13(9), e0203622-e0203622 (2018-09-08)
Spermatogenesis in many species including Drosophila melanogaster is accompanied by major reorganisation of chromatin in post-meiotic stages, involving a nearly genome-wide displacement of histones by protamines, Mst77F and Protamine-like 99C. A proposed prerequisite for the histone-to-protamine transition is massive histone
Louis Philip Benoit Bouvrette et al.
RNA (New York, N.Y.), 24(1), 98-113 (2017-10-29)
Cells are highly asymmetrical, a feature that relies on the sorting of molecular constituents, including proteins, lipids, and nucleic acids, to distinct subcellular locales. The localization of RNA molecules is an important layer of gene regulation required to modulate localized
Stefanie M K Gärtner et al.
European journal of cell biology, 94(1), 46-59 (2014-12-04)
Spermatogenesis is accompanied by a remarkable reorganization of the chromatin in post-meiotic stages, characterized by a near genome-wide displacement of histones by protamines and a transient expression of transition proteins. In Drosophila, the transition-protein-like protein Tpl94D contains an HMG-box domain
Ahmed Sabri et al.
PloS one, 6(4), e18847-e18847 (2011-04-29)
Aphidius ervi (Hymenoptera: Braconidae) is an entomophagous parasitoid known to be an effective parasitoid of several aphid species of economic importance. A reduction of its production cost during mass rearing for inundative release is needed to improve its use in
Yuankai Dong et al.
Genetics, 215(1), 89-101 (2020-03-18)
BEAF (Boundary Element-Associated Factor) was originally identified as a Drosophila melanogaster chromatin domain insulator-binding protein, suggesting a role in gene regulation through chromatin organization and dynamics. Genome-wide mapping found that BEAF usually binds near transcription start sites, often of housekeeping
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