RAB0874

Porcine INS / Insulin ELISA Kit

• UNSPSC-Code: 41116158
• NACRES: NA.32

Eigenschaften

• Speziesreaktivität: porcine
• Verpackung: kit of 96 wells (12 strips x 8 wells)
• Methode(n): ELISA: suitable
• Aufnahme: sample type plasma; sample type cell culture supernatant(s); sample type serum
• assay range: inter-assay cv: <12%; intra-assay cv: <10%; sensitivity: 4 μIU/mL; standard curve range: 4.69 μIU/ml-300 μIU/ml
• Nachweisverfahren: colorimetric
• Versandbedingung: wet ice
• Lagertemp.: −20°C
• Angaben zum Gen: Porcine ... INS(397415)

Beschreibung

Allgemeine Beschreibung

The antibody reacts specifically against insulin by RIA and immunocytochemistry.[1][2][3] It exhibits cross-reactivity with human proinsulin.[3] The antibody binds to insulin with an affinity constant of 8.8 × 10⁹ M^-1 in RIA.[1][2] The insulin gene is mapped to human chromosome 11p15.5.[4]
Human insulin is made up of 51 amino acids. It consists of two peptide chains, 21 amino acids make up A-chain and chain B contains 31 amino acids. The two chains are linked together by a disulfide bond, to form a classical insulin fold with three α helices, chain A having two and one in chain B.[5]

Anwendung

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem./physiol. Wirkung

Abnormal insulin secretion or function, leads to diabetes mellitus, obesity and other carbohydrate metabolism disorders.[6][7] Insulin is known to stimulate cell division. The biological effects of insulin are mediated by insulin receptor and it integrates major signaling pathways.[6]

Sonstige Hinweise

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit-Komponenten auch einzeln erhältlich

• RABTMB3 ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)
• RABSTOP3 ELISA Stop Solution (Item I)
• RABWASH4 20X Wash Buffer (Item B)

Sicherheitshinweise

• Piktogramme: GHS05
• Signalwort: Warning
• H-Sätze: H290
• P-Sätze: P234 - P390
• Gefahreneinstufungen: Met. Corr. 1
• Lagerklassenschlüssel: 8A - Combustible corrosive hazardous materials
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable