RAB0477

Mouse Tumor Necrosis Factor α ELISA Kit

for serum, plasma and cell culture supernatant

• Synonym(e): Cachectin, TNF-alpha, Tumor necrosis factor alpha
• UNSPSC-Code: 41116158
• NACRES: NA.32

Eigenschaften

• Speziesreaktivität: mouse
• Verpackung: kit of 96 wells (12 strips x 8 wells)
• Methode(n): ELISA: suitable; capture ELISA: suitable
• Aufnahme: sample type serum; sample type cell culture supernatant(s); sample type plasma
• assay range: inter-assay cv: <12%; intra-assay cv: <10%; sensitivity: 60 pg/mL; standard curve range: 93.75-6000 pg/mL
• Nachweisverfahren: colorimetric
• Versandbedingung: wet ice
• Lagertemp.: −20°C
• Angaben zum Gen: mouse ... Tnf(21926)

Beschreibung

Allgemeine Beschreibung

The Mouse TNF-α ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse TNF-α in serum, plasma and cell culture supernatants.

Tumor Necrosis Factor α (TNF-α) is a proinflammatory cytokine. It is a transmembrane protein and the final protein is devoid of the extracellular domain. TNF-α-converting enzyme (TACE) catalyzes the formation of the soluble homotrimer TNF-α.

Immunogen

Recombinant Mouse TNF-α

Anwendung

Mouse Tumor Necrosis Factor α ELISA Kit has been used in measuring TNF-αin MC3T3-E1 murine preosteoblastic calvarial cell lines, rat serum, mice liver and kidney samples.

Biochem./physiol. Wirkung

Tumor Necrosis Factor α (TNF-α) elicits inflammatory and immuno-regulatory functionality. It mediates its effects via its receptors TNFR1 and TNFR2.

Sonstige Hinweise

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit-Komponenten auch einzeln erhältlich

• RABELADA ELISA 1X Assay/Sample Diluent Buffer A (Item D1)
• RABELADB ELISA 5X Assay/Sample Diluent Buffer B (Item E1)
• RABSTOP3 ELISA Stop Solution (Item I)
• RABTMB3 ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)
• RABWASH4 20X Wash Buffer (Item B)

Sicherheitshinweise

• Piktogramme: GHS05
• Signalwort: Warning
• H-Sätze: H290
• P-Sätze: P234 - P390
• Gefahreneinstufungen: Met. Corr. 1
• Lagerklassenschlüssel: 8A - Combustible corrosive hazardous materials