RAB0364

Human MMP-13 ELISA Kit

for serum, plasma, cell culture supernatant and urine

• NACRES: NA.32
• UNSPSC Code: 41116158
• Gene information: human ... MMP13(4322)
• Input: sample type cell culture supernatant(s); sample type urine; sample type plasma; sample type serum
• Species reactivity: human
• Storage temp.: −20°C
• Shipped in: wet ice

Eigenschaften

• species reactivity: human
• packaging: kit of 96 wells (12 strips x 8 wells)
• technique(s): ELISA: suitable, capture ELISA: suitable
• input: sample type cell culture supernatant(s); sample type urine; sample type plasma; sample type serum
• assay range: inter-assay cv: <12%; intra-assay cv: <10%; sensitivity: 6 pg/mL; standard curve range: 8.23-6000 pg/mL
• detection method: colorimetric
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... MMP13(4322)

Beschreibung

General description

The Human MMP-13 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human MMP-13 in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human MMP13

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

MMP13 (matrix metalloproteinase 13) functions against different types of ECM (extracellular matrix) components. It gets activated by and activates multiple MMPs and hence, has a central role in the MMP cascade. This protein is frequently expressed in colorectal cancer (CRC) and this expression is linked with poor survival in CRC. MMP13 expression is absent in normal breast tissue, but is present in breast carcinoma suggesting its role in tumorigenesis. The expression of this protein can serve as a marker for tumor invasiveness, as its expression is induced during metastasis of multiple types of cancers, including squamous cell carcinomas of the head and neck and this expression is usually linked to poor prognosis. Missense mutation in this gene results in the autosomal dominant disorder called Missouri type of human spondyloepimetaphyseal dysplasia (SEMDMO), which is characterized by abnormal growth and modeling of vertebrae and long bones.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit-Komponenten auch einzeln erhältlich

• RABELADE

  ELISA 5X Assay/Sample Diluent Buffer E (Item E2)

  SDB
• RABSTOP3

  ELISA Stop Solution (Item I)

  SDB
• RABTMB3

  ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)

  SDB
• RABWASH4

  20X Wash Buffer (Item B)

  SDB

Sicherheitshinweise

• Piktogramme: GHS05
• Signalwort: Warning
• Gefahrencodes: H290
• Sicherheitshinweise: P234 - P390
• Hazard Classifications: Met. Corr. 1
• Lagerklasse: 12 - Non Combustible Liquids
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable