RAB0028

Human BMP2 ELISA Kit

for serum, plasma, cell culture supernatant and urine

• Synonym(e): BMP-2, Bone morphogenetic protein 2
• UNSPSC-Code: 41116158
• NACRES: NA.32

Eigenschaften

• Speziesreaktivität: human
• Verpackung: kit of 96 wells (12 strips x 8 wells)
• Methode(n): ELISA: suitable; capture ELISA: suitable
• Aufnahme: sample type serum; sample type cell culture supernatant(s); sample type urine; sample type plasma
• assay range: inter-assay cv: <12%; intra-assay cv: <10%; sensitivity: 45 pg/mL; standard curve range: 46.88-3000 pg/mL
• Nachweisverfahren: colorimetric
• Versandbedingung: wet ice
• Lagertemp.: −20°C
• Angaben zum Gen: human ... BMP2(650)

Beschreibung

Allgemeine Beschreibung

BMP2 (bone morphogenetic protein 2) is encoded by the gene mapped to human chromosome 20p12.3. The encoded protein belongs to transforming growth factor-β (TGFβ) superfamily. BMP-2 is expressed in heart, lung, and dorsal aorta.

The Human BMP-2 ( Bone morphogenetic proteins-2) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme- linked immunosorbent assay for the quantitative measurement of human BMP-2 in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human BMP2

Anwendung

For research use only. Not for use in diagnostic procedures.
Human BMP2 (bone morphogenetic protein 2) ELISA kit has been used for measuring concentrations of BMP in conditioned mouse organoid media.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem./physiol. Wirkung

BMP2 (bone morphogenetic protein 2) plays a vital role in the activation of the autonomic lineage-specific basic–helix-loop-helix protein MASH1 (mammalian achaete scute homolog-1) and in the autonomic neurogenesis in neural crest stem cells in vitro. During bone repair, BMP-2 controls the expression of Runt-related transcription factor 2 (Runx2) and Osterix (Osx), thereby enhancing migration, proliferation and osteogenic differentiation of mesenchymal stem cells. In addition to its osteogenic activity, BMP-2 also plays an important role in cardiac morphogenesis. Recombinant human bone morphogenetic protein-2 (rhBMP-2) plays an essential role in pathogenesis of oral squamous cell carcinomas. Mutation in BMP-2 gene is associated with osteoporosis.

Sonstige Hinweise

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit-Komponenten auch einzeln erhältlich

• RABELADA ELISA 1X Assay/Sample Diluent Buffer A (Item D1)
• RABELADB ELISA 5X Assay/Sample Diluent Buffer B (Item E1)
• RABSTOP3 ELISA Stop Solution (Item I)
• RABTMB3 ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)
• RABWASH4 20X Wash Buffer (Item B)

Sicherheitshinweise

• Piktogramme: GHS05
• Signalwort: Warning
• H-Sätze: H290
• P-Sätze: P234 - P390
• Gefahreneinstufungen: Met. Corr. 1
• Lagerklassenschlüssel: 8A - Combustible corrosive hazardous materials