P0157

Phosphatase, sauer aus Kartoffeln

ammonium sulfate suspension, ≥200 units/mg protein (biuret)

• Synonym(e): (Ortho-Phosphor-Monoester-Phosphohydrolase, Optimum im sauren Bereich), Saure Phosphatase aus Kartoffeln
• CAS-Nummer: 9001-77-8
• EC-Nummer: 3.1.3.2 (BRENDA, IUBMB)
• EG-Nummer: 232-630-9
• MDL-Nummer: MFCD00131847
• UNSPSC-Code: 12352204

Eigenschaften

• Form: ammonium sulfate suspension
• Spezifische Aktivität: ≥200 units/mg protein (biuret)
• Mol-Gew.: 111 kDa
• Fremdaktivität: Apyrase (ATPase) ≤5% of base activity
• Lagertemp.: 2-8°C
• InChI: 1S/C6H10O2/c1-3-4-8-5-6(2)7/h1,6-7H,4-5H2,2H3
• InChIKey: GZCWLCBFPRFLKL-UHFFFAOYSA-N

Beschreibung

Allgemeine Beschreibung

Acid phosphatase from potatoes is a 111 kDa diner consisting of two subunits at 41 and 35 kDa. This phosphatase has also been shown to cleave DNA.

Anwendung

Phosphatase acid from potato has been used in a study to develop a method of efficient enzymatic hydrolysis of polyprenyl pyrophosphates. It has also been used in a study to investigate the kinetics of the hydrolysis of sodium p-nitrophenylphosphate and other phosphoric acid monoesters.

Biochem./physiol. Wirkung

Acid phosphatases (APase) are a family of enzymes that non-specifically catalyze the hydrolysis of monoesters and anhydrides of phosphoric acid to produce inorganic phosphate at an optimum pH of 4 to 7.

Einheitendefinition

One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 4.8 at 37 °C.

Physikalische Form

Suspension in 1.8 M (NH₄)₂SO₄, 10 mM MgCl₂, pH 5.5

Sicherheitshinweise

• Piktogramme: GHS08
• Signalwort: Danger
• H-Sätze: H334
• P-Sätze: P261 - P284 - P501
• Gefahreneinstufungen: Resp. Sens. 1
• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)