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Merck

P0029

Sigma-Aldrich

Protease aus Bacillus sp.

Synonym(e):

Protamex®

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50 G
CHF 195.00
250 G
CHF 413.00

CHF 195.00


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50 G
CHF 195.00
250 G
CHF 413.00

About This Item

EC-Nummer:
3.4.21.14
EG-Nummer:
MDL-Nummer:
UNSPSC-Code:
12352204
NACRES:
NA.54

CHF 195.00


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Biologische Quelle

Bacillus sp.

Form

granular

enzyme activity

>1.5 AU-N/g

Lagertemp.

2-8°C

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Allgemeine Beschreibung

Protease from Bacillus sp. is extensively useful in pharmaceutical, leather, food and waste processing industry. It is widely used in manufacturing protein hydrolysates.[1] Analysis of Bacillus protease stability and activity showed that the enzyme was active at all pH values from 5.0-11.0 with 100% activity at 8.0. Optimal pH for protein stability was determined to be at 7.0 while the optimal temperature for stability was at 60°C with 95% activity maintained.[2]

Anwendung

Protease from Bacillus sp. has been used:
  • to evaluate protease inhibitor activity[3]
  • in high yields in batch fermentation using a response surface methodology[4]
  • in a study to investigate a biosensor to detect a protease biomarker from Bacillus licheniformis[5]

Hinweis zur Analyse

minimum activity 1.5 AU-N/G solid

Rechtliche Hinweise

Protamex is a registered trademark of Novozymes Corp.

Piktogramme

Health hazardExclamation mark

Signalwort

Danger

Gefahreneinstufungen

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Zielorgane

Respiratory system

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 2

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


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Die Dokumentenbibliothek aufrufen

Characterization of protein hydrolysates and lipids obtained from black scabbardfish (Aphanopus carbo) by-products and antioxidative activity of the hydrolysates produced.
Batista, I., et al
Process. Biochem., 45(1), 18-24 (2010)
Production and properties of an extracellular protease from thermophilic Bacillus sp
<BIG>Nascimentol W, et al.</BIG>
Brazilian Journal of Microbiology, 35, 91-96 (2004)
Sumant Puri et al.
Current microbiology, 44(4), 286-290 (2002-03-23)
High yields (1939 U/ml) of an alkaline protease were obtained in batch fermentation of a Bacillus sp. using a response surface methodology. The interaction of four variables, viz., starch, peptone, incubation time, and inoculum density, suggested inoculum density to be
Some properties of extracellular protease from Bacillus licheniformis LBBL-11 isolated from ?iru?, a traditionally fermented African locust bean condiment.
Olajuyigbe, FM.
African Journal of Biochemistry Research, 2, 206-210 (2008)
Hypocholesterolemic effect of compounded freshwater clam protein hydrolysate and Gracilaria
Lin, Y-H, et al
Food Chemistry, 123(2), 395-399 (2010)

Questions

1–2 of 2 Questions  
  1. Podrían indicarme las temperaturas y pH óptimos de trabajo de esta proteasa

    1 answer
    1. As mentioned in the 'DESCRIPTION' section, the optimal pH for protein stability was determined to be at 7.0 while the optimal temperature for stability was at 60°C with 95% activity maintained.

      Helpful?

  2. What is the composition (or protein content) of this protease?

    1 answer
    1. This is a product of Novozymes. The composition is considered proprietary, however, it is a blend of aggressive endo-proteases.

      See the link below to a publication that may be helpful:
      https://www.sciencedirect.com/science/article/pii/S2772502222000683

      Helpful?

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