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MP0025

Sigma-Aldrich

Venor™ GeM Mycoplasma Detection Kit, PCR-based

Synonym(e):

PCR mycoplasma detection kit

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About This Item

UNSPSC-Code:
12352207
NACRES:
NA.75

Verwendung

 kit sufficient for 25 tests

Qualitätsniveau

200

Verpackung

pkg of 1 kit

Lagerbedingungen

dry at room temperature

Methode(n)

PCR: suitable

Anwendung(en)

detection
microbiology

Lagertemp.

2-8°C

Verwandte Kategorien

Allgemeine Beschreibung

The Venor™ GeM Mycoplasma Detection Kit utilizes the polymerase chain reaction (PCR), which was established as the method of choice for highest sensitivity in the detection of Mycoplasma and Acholeplasma contamination in cell cultures and other cell culture derived biologicals. Detection requires as little as 1–5fg of mycoplasma DNA corresponding to 2–5 mycoplasma per sample volume. The primer set is specific to the highly conserved rRNA operon, or more specifically, the 16S rRNA coding region in the mycoplasma genome. This allows for detection of all Mycoplasma, Acholeplasma and Ureaplasma species tested so far, which are usually encountered as contaminants in cell cultures. Eukaryotic and bacterial DNA are not amplified by this kit.

Anwendung

The PCR-based Venor™ GeM Mycoplasma Detection Kit employs PCR technology for rapid and reliable detection of mycoplasma DNA in cell cultures and virus stocks.

Komponenten

Does not include Taq Polymerase. Optimized for use with D9307, Taq DNA Polymerase

Lagerung und Haltbarkeit

The kit components are stable during shipping at ambient temperature. Upon receipt, store at 2–8 °C. After rehydration of the primer/nucleotide mix, the positive control, and the internal control, store below –20 °C and avoid repeated freezing and thawing. For repeated testing of low sample numbers, primer/nucleotide mix and controls should be aliquoted after rehydration. By following these recommendations, the kit is stable until the expiration date stated on the label.

Rechtliche Hinweise

Venor is a trademark of Minerva Biolabs GmbH

Nur Kit-Komponenten

Produkt-Nr.
Beschreibung
  • Positive Control
  • Negative Control
  • PCR 10X Reaction Buffer 500 mL/vial
  • Primer/Nucleotide Mix 1 mL/vial

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 3

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Die Dokumentenbibliothek aufrufen

Mycoplasma detection in a historical arbovirus repository: Commercial kit comparison and implications for improved repository management
Russell BJ, et al.
Journal of Virological Methods (2020)
Anbarasu Kannan et al.
Scientific reports, 7, 46102-46102 (2017-04-07)
Human papilloma virus-16 (HPV-16) associated oropharyngeal cancer (HPVOPC) is increasing alarmingly in the United States. We performed whole genome sequencing of a 44 year old, male HPVOPC subject diagnosed with moderately differentiated tonsillar carcinoma. We identified new somatic mutation in
Evolution of Hoxa11 regulation in vertebrates is linked to the pentadactyl state
Kherdjemil Y, et al.
Nature (2016)
Julie V Philley et al.
Journal of cellular physiology, 231(6), 1364-1374 (2015-11-05)
Mitochondria (mt) encoded respiratory complex-I (RCI) mutations and their pathogenicity remain largely unknown in prostate cancer (PCa). Little is known about the role of mtDNA loss on mt integrity in PCa. We determined mtDNA mutation in human and mice PCa
Anthony J Bainor et al.
Cancer research, 77(19), 5339-5348 (2017-08-16)
Distinguishing between indolent and aggressive prostate adenocarcinoma remains a priority to accurately identify patients who need therapeutic intervention. SIN3B has been implicated in the initiation of senescence
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Protokolle

Optimized PCR-based Detection of Mycoplasma

Mycoplasma contamination of cell cultures is a serious issue impacting cell model validity. PCR testing for mycoplasma is an inexpensive, sensitive, and specific method for detecting contamination.

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