L8525

Lipase aus Candida rugosa

lyophilized powder, ≥40,000 units/mg protein

• Synonym(e): Lipase AY 30 Amano, Triacylglycerol-acylhydrolase, Triacylglycerol-lipase
• CAS-Nummer: 9001-62-1
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-619-9
• EG-Nummer: 3.1.1.3 (BRENDA, IUBMB)
• MDL number: MFCD00131509
• Specific activity: ≥40,000 units/mg protein
• Biological source: fungus (Candida rugosa)

Eigenschaften

• biological source: fungus (Candida rugosa)
• Quality Segment: 200
• form: lyophilized powder
• specific activity: ≥40,000 units/mg protein
• composition: Protein, ≥4.0%
• foreign activity: α-amylase ≤0.05 unit/mg solid, protease ≤0.001 units/mg solid
• storage temp.: 2-8°C
• InChI: 1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;
• InChI key: QWZUIMCIEOCSJF-CHHCPSLASA-N

Beschreibung

General description

Microbes of plant and animal basis mainly produce lipase enzyme. It can also be produced by marine organisms.

Application

Lipase from C. rugosa is used to catalyze the synthesis of flavor esters such as pentyl propanoate and butyl butanoate used in food enhancement.

Lipase from Candida rugosa has been used:

• in the hydrolysis of loaded polycaprolactone–20% tricalcium phosphate (PCL–TCP) composites
• in adsorption of proteins in emulsan/alginate beads and in enzyme assays
• to catalyse methanolysis and hydrolysis reactions

Lipasen werden in der Industrie zum Auflösen von chiralen Verbindungen und zur Umesterung bei der Herstellung von Biodiesel verwendet.

Lipase from Candida rugosa has been used in a study to assess the influence of phase segregation on enzyme hydrolytic activity. [1]

Biochem/physiol Actions

Lipases are used in detergent, food, leather, pharmaceutical and diary industries. It has several characteristics like substrate specificity, stereospecificity and regioselectivity. It also participates in various bioconversion reactions like, hydrolysis, esterification, acidolysis and aminolysis.

Tri-, Di- und Monoglyceride werden hydrolysiert (in abnehmender Reihenfolge der Rate).

Tri-, Di- und Monoglyceride werden hydrolysiert (in abnehmender Reihenfolge der Rate).

Lipasen katalysieren die Hydrolyse von Triacylglycerinen zu Glycerin und freien Fettsäuren.

Analysis Note

Protein determined by biuret.

Other Notes

One unit will hydrolyze 1.0 microequivalent of fatty acid from a triglyceride in 1 hr at pH 7.2 at 37 °C using olive oil (30 minute incubation).

Sicherheitshinweise

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)