I4401

Interferon-αA/D human

≥95%, recombinant, expressed in E. coli, buffered aqueous solution, suitable for cell culture

• Synonym(e): IFN-αA/D
• MDL-Nummer: MFCD01635116
• UNSPSC-Code: 12352202
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: human
• Qualitätsniveau: 200
• Rekombinant: expressed in E. coli
• Assay: ≥95%
• Form: buffered aqueous solution
• Mol-Gew.: 19 kDa
• Verpackung: pkg of 100K units
• Methode(n): cell culture | mammalian: suitable
• Verunreinigungen: endotoxin, tested
• Farbe: colorless
• UniProt-Hinterlegungsnummer: P01563
• Versandbedingung: dry ice
• Lagertemp.: −70°C
• Angaben zum Gen: human ... IFNA2(3440)

Beschreibung

Anwendung

Interferon-αA/D human has been used:

• for identification of coronavirus replicase inhibitors[1]
• in combination with ACH-806 [1-(4-pentyloxy-3-trifluoromethylphenyl)-3-(pyridine-3-carbonyl)thiourea] to study the effect on hepatitis C virus (HCV) replication[2]
• in combination with vitamin D to check reduction in HCV protein production in cell culture[3]

Biochem./physiol. Wirkung

Interferon-αA/D (Bg/II), also known as Universal Type I Interferon, is an α interferon hybrid that crosses the species barrier. The Bg/II denotes the restriction enzyme site from which this hybrid is constructed from recombinant human interferons αA and αD. Interferon-αA/D has been proven to be active on many mammalian cells. It can substitute for human, monkey, mouse, bovine, rat, cat, dog, rabbit, sheep, goat, horse, pig or hamster type I interferon (α, β, ω and τ). Type I Interferons are a closely related family of 165-172 amino acid proteins that are produced by leukocytes (α subtypes), fibroblasts (β subtypes), lymphocytes (ω subtypes), and ruminant embryos (τ subtypes). This cytokine has anti-viral, anti-proliferative, immunoregulatory, and proinflammatory activities.

This cytokine has anti-viral, anti-proliferative, immunoregulatory, and proinflammatory activities.

Physikalische Form

Solution in phosphate buffered saline containing 0.1% bovine serum albumin

Hinweis zur Analyse

The biological activity is determined in the cytopathic effect inhibition assay using MDBK cells with vesicular stomatitis virus (VSV).[4][5] A cytopathic effect of 50% is produced with 1 unit/ml interferon. The units are determined with respect to NIH international standard reference for human interferonαA.[6]

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves