E2884

Esterase aus Schweineleber

ammonium sulfate suspension, ≥150 units/mg protein (biuret)

• Synonym(e): Carboxyl esterase, Carboxylester-Hydrolase, PLE
• CAS-Nummer: 9016-18-6
• UNSPSC Code: 12352204
• NACRES: NA.54
• EG-Nummer: 3.1.1.1 (BRENDA, IUBMB)
• MDL number: MFCD00131030
• Specific activity: ≥150 units/mg protein (biuret)
• Biological source: Porcine liver

Eigenschaften

• biological source: Porcine liver
• Quality Segment: 300
• form: ammonium sulfate suspension
• specific activity: ≥150 units/mg protein (biuret)
• mol wt: 168 kDa
• storage temp.: 2-8°C

Beschreibung

General description

Esterase, also known as carboxyl ester hydrolase is a member of the hydrolase superfamily of enzymes.

Application

The enzyme from Sigma has been used to develop an alternative gel-based protocol for screening for bead-bound catalytic activity in aqueous media. The product has also been used to investigate the properties of the compound L-valine-3-{8-[(E)-2-[3-methoxyphenyl)ethenyl]-7-methyl-1-propargylxanthine-3-yl}propyl ester hydrochloride (MSX-4, an aminoacid ester prodrug of the adenosine A2A receptor antagonist MSX-2).

Esterase aus der Schweineleber wird zur Katalysierung der Hydrolyse von Pentaacetylcatechin und Epicatechin bei pharmazeutischen sowie industriellen Anwendungen verwendet.

Esterase aus der Schweineleber wird häufig für kinetische Auflösungen und die asymmetrische Synthese in der organischen Chemie verwendet.

Esterase from porcine liver has been used to unmask the fluorescence in ultraviolet-visible spectroscopy.

Biochem/physiol Actions

Pig liver esterase catalyzes enantioselective conversion of an ester to a carboxylic acid. The molecular weight is found to be 168 kDa. It is a serine enzyme with two active sites on each molecule, which dissociates into active half-molecules in the presence of dilute acid or concentrated salts.

Esterase wirkt auf wasserlösliche Carboxylester ein, die kurzkettige Fettsäuren enthalten. Ihre Funktionalität wird auf die katalytische Triade Ser, His und Asp/Glu zurückgeführt.

Esterase enzyme of bacteria and fungi seems to have several industrial applications. It is capable of catalyzing the hydrolysis and synthesis of ester bonds.

Physical form

Suspension in 3.2 M (NH₄)₂SO₄, pH 8

Other Notes

One unit will hydrolyze 1.0 μmole of ethyl butyrate to butyric acid and ethanol per min at pH 8.0 at 25 °C.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)