E1257

Epidermaler Wachstumsfaktor aus Maussubmaxillardrüse

lyophilized powder, BioReagent, suitable for cell culture

• Synonym(e): EGF, Epidermis-Wachstumsfaktor
• CAS-Nummer: 62229-50-9
• EG-Nummer: 263-468-7
• MDL-Nummer: MFCD01634807
• UNSPSC-Code: 12352202
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: mouse submaxillary glands
• Qualitätsniveau: 200
• Produktlinie: BioReagent
• Assay: ≥98% (SDS-PAGE)
• Form: lyophilized powder
• Wirksamkeit: 0.02-2.0 ng/mL EC₅₀
• Verpackung: pkg of 0.1 mg
• Lagerbedingungen: avoid repeated freeze/thaw cycles
• Methode(n): cell culture | mammalian: suitable
• Verunreinigungen: endotoxin, tested
• Farbe: white
• UniProt-Hinterlegungsnummer: P01132
• Lagertemp.: 2-8°C
• Angaben zum Gen: mouse ... Egf(13645)

Beschreibung

Allgemeine Beschreibung

Epidermal growth factor (EGF) is the member of EGF-like family of growth factors, that binds to EGF receptor.[1] It is majorly expressed in kidney,[2] parotid gland and salivary gland.[3]

Anwendung

Epidermal Growth Factor from murine submaxillary gland has been used

• in studying its effects on the development of caustic esophageal stricture in rats[4]
• for preparation of MCF-10A cell line[5]
• for the culture of primary hair follicle stem cells from arbas cashmere goat (gHFSCs) and adhesion of hair follicles (HF)[6]

Biochem./physiol. Wirkung

Epidermal growth factor (EGF) blocks gastric acid and pepsin secretion and increases gastrointestinal cell proliferation.[7] EGF stimulates proliferation and migration in bone marrow stromal cells (BMSC), that gives rise to osteoblasts, chondrocytes and adipocytes.[8] It functions as a strong mitogenic factor for various cultured cells. Mutations in this gene is linked to hypomagnesemia.[1] EGF plays a major role in the maintenance of hair and skin development.[9]

Physikalische Form

Lyophilized from 0.2 μm filtered solution in ammonium acetate buffer.

Angaben zur Herstellung

Purified by gel filtration and ion-exchange chromatography.

Hinweis zur Analyse

The biological activity is measured in a proliferation assay using BALB/MK cells.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)