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D6665
2′,7′-Dichlorfluoreszein
~90% purity (TLC) (85- 100% purity), powder
Synonym(e):
Dichlorofluorescein; Fluorescein 27
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| Ihnen/SKU | Verfügbarkeit | Preis |
|---|---|---|
5 g | Warenkorb auf Verfügbarkeit prüfen | CHF 143.00 |
25 g | Warenkorb auf Verfügbarkeit prüfen | CHF 632.00 |
Über diesen Artikel
Empirische Formel (Hill-System):
C20H10Cl2O5
CAS-Nummer:
Molekulargewicht:
401.20
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
EC Number:
200-968-6
MDL number:
Beilstein/REAXYS Number:
58009
Technischer Dienst
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Unterstützung erhaltenProduktname
2′,7′-Dichlorfluoreszein, suitable for use as an indicator in chloride titration, ~90% (TLC), powder
Quality Segment
assay
~90% (TLC)
form
powder
technique(s)
titration: suitable
mp
280 °C (dec.) (lit.)
solubility
ethanol: 25 mg/mL
density
0.79 g/cm3
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
Oc1cc2Oc3cc(O)c(Cl)cc3C4(OC(=O)c5ccccc45)c2cc1Cl
InChI
1S/C20H10Cl2O5/c21-13-5-11-17(7-15(13)23)26-18-8-16(24)14(22)6-12(18)20(11)10-4-2-1-3-9(10)19(25)27-20/h1-8,23-24H
InChI key
VFNKZQNIXUFLBC-UHFFFAOYSA-N
General description
2′,7′-Dichlorofluorescein is an oxidation-sensitive fluorescent probe. 2′7′-dichlorofluorescein diacetate is oxidized to 2′7′-dichlorofluorescein in the presence of ROS (reactive oxygen species).citation
Application
2′,7′-Dichlorofluorescein has been used as an oxidation-sensitive fluorescent probe to measure ROS (reactive oxygen species) formation in cells.[1]
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Dieser Artikel | |||
|---|---|---|---|
| technique(s) titration: suitable | technique(s) titration: suitable | technique(s) - | technique(s) - |
| form powder | form powder | form solid | form powder |
| solubility ethanol: 25 mg/mL | solubility alcohol: passes test | solubility - | solubility - |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) - | application(s) - |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 200 |
| storage temp. room temp | storage temp. room temp | storage temp. - | storage temp. −20°C |
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Lagerklasse
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
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What is the Department of Transportation shipping information for this product?
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In what types of applications can 2´,7´-Dichlorofluorescein be used?
1 answer-
Some applications for use of 2´,7´-Dichlorofluorescein are listed below. This product can be used for the determination of carbohydrates by fluorescence densitometry after TLC. See G. Gübitz et al., J. Chromatogr., 117, 337, (1976). 2´,7´-Dichlorofluorescein as also been used in the determination of H2O2 by chemiluminescence. See the following references by H. Watanabe et al. Anal. Sci. 2, 461, (1986) and T. Segawa et al. Anal. Sci. 6, 763, (1990). This product has also been used as a fluorescent additive used in indirect fluorimetric detection in OTC-HPLC. See W.D. Pfeffer and E.S. Yeung, J. Chromatogr., 506, 401, (1990).
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What can be used to make a solution of 2´,7´-dichlorofluorescein?
1 answer-
2´,7´-Dichlorofluorescein is soluble at 25 mg/mL absolute ethanol with heating. 2´,7´-Dichlorofluorescein is also soluble in water at 0.1 mg/mL.
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What excitation /emission wavelengths are best for fluorescence detection of 2´,7´-Dichlorofluorescein?
1 answer-
2´,7´-Dichlorofluorescein can be detected at an excitation wavelength at 504 nm and emission wavelength at 529 nm in 0.1 M Tris pH 8.0.
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